MABF244

Anti-PAR-1, clone ATAP2 Antibody

clone ATAP2, from mouse

• Sinonimo/i: Proteinase-activated receptor 1, PAR-1, Coagulation factor II receptor, Thrombin receptor
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: ATAP2, monoclonal
• Reattività contro le specie: human, mouse
• tecniche: flow cytometry: suitable; immunocytochemistry: suitable; immunofluorescence: suitable; western blot: suitable
• Isotipo: IgG1κ
• N° accesso NCBI: NP_001983
• N° accesso UniProt: P25116
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... F2R(2149)

Descrizione

Descrizione generale

The protein named Proteinase-activated receptor 1 (PAR-1/PAR1) or Coagulation factor II receptor, or Thrombin receptor and encoded by the human gene F2R/CR2R/PAR1/TR is a high affinity receptor for activated thrombin and part of the innate immune system response driven by proteinase-activated receptors (PARs). PARs are G protein-coupled receptors that transmit cellular responses to extracellular proteases and have important functions in vascular physiology, development, inflammation, and cancer progression. PAR1 is important for platelet activation and vascular development. PAR1 expression is limited to platelets and vascular endothelial cells and is localized to the cell membrane. Interestingly too, even the signal peptide of PAR1 (which guides the protein to the membrane) plays a role in cell signaling as if forms the intracellular angiogenesis inhibitor peptide Parstatin.

Immunogeno

Linear peptide corresponding to human Par-1.

Applicazioni

Flow Cytometry Analysis: 4 µg from a representative lot detected Par-1 in 1X10E6 human thrombocytes (platelets).

Flow Cytometry Analysis: A representative lot from an independent laboratory detected Par-1 in CHRF-288 and HEL cells (Hoxie, J. A., et al. (1993). J Biol Chem. 268(18):13756-13763.).

Western Blotting Analysis: A representative lot from an independent laboratory detected Par-1 in human platelet membrane cell lysate (Brass, L. F., et al. (1992). J Biol Chem. 267(20):13795-13798.).

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Par-1 in CHRF-288 and HEL cells (Hoxie, J. A., et al. (1993). J Biol Chem. 268(18):13756-13763.).

Immunofluorescence Analysis: A representative lot from an independent laboratory detected Par-1 in CHRF-288 cells (Brass, L. F., et al. (1994). J Biol Chem. 269(4):2943-2952.).

This Anti-PAR-1 antibody, clone ATAP2 is validated for use in western blotting, flow cytometry, ICC & immunofluorescence for the detection of PAR-1.

Qualità

Evaluated by Western Blotting in mouse lymph node tissue.

Western Blotting Analysis: 1 µg/mL of this antibody detected Par-1 in 10 µg of mouse lymph node tissue.

Descrizione del bersaglio

~50 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Stato fisico

Format: Purified

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable