MABF2154

Anti-Ricin B Chain Antibody, clone RBC11

clone RBC11, from mouse

• Sinonimo/i: RBC
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: RBC11, monoclonal
• Reattività contro le specie: Ricinus communis
• Confezionamento: antibody small pack of 25 μg
• tecniche: ELISA: suitable; flow cytometry: suitable
• Isotipo: IgG2bκ
• N° accesso UniProt: D0VFC4
• modifica post-traduzionali bersaglio: unmodified

Descrizione

Descrizione generale

Ricin is a dimeric toxin derived from Ricinus communis (Caster bean). It is composed of a sugar-binding subunit (B chain) that attached to cell surface receptors and facilitate the entry of toxin into the target cell and a second subunit (A chain), which has glycosidase activity that inactivates ribosomes by depurinating a single adenosine residue from an exposed loop of the 28S ribosomal RNA. The toxic effects of Ricin are attributed to the A chain. Ricin B chain is shown to be a lectin that binds to galactosides found at the cell surface in a non-cooperative manner. It has two Ricin-B type lectin domains (aa 7-134 and 137-261). The mode of galactose binding is reported to be nearly identical at the two sites. Clone RBC11 is shown to block binding of Ricin to the cell. (Ref.: Maddaloni, M., et al. (2004). J. Immunol. 172; 6221-6228; Rutenber, E., et al. (1987). Nature. 326(6113):624-626).

Specificità

Clone RBC 11 binds to Ricin B chain.

Immunogeno

Purified Ricin B chain.

Applicazioni

Anti-Ricin B Chain, clone RBC11, Cat. No. MABF2154, is a mouse monoclonal antibody that detects Ricin B chain and has been tested for use in ELISA and Flow Cytometry.

Flow Cytometry Analysis: A representative lot detected Ricin B chain in Flow Cytometry applications (Maddaloni, M., et. al. (2004). J Immunol. 172(10):6221-8).

ELISA Analysis: A representative lot detected Ricin B chain in ELISA applications (Maddaloni, M., et. al. (2004). J Immunol. 172(10):6221-8; Simon, S., et. al. (2015). Toxins (Basel) 7(12):4967-86).

Research Category
Inflammation & Immunology

Qualità

Evaluated by ELISA with Ricin B chain.

ELISA Analysis: Various dilutions of this antibody detected Ricin B chain in an indirect ELISA.

Descrizione del bersaglio

28.90 kDa calculated.

Stato fisico

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable