MABE328
Anti-MeCP2 Antibody, clone 4H7
clone 4H7, from rat
Sinonimo/i:
Methyl-CpG-binding protein 2, MeCp-2 protein, MeCp2
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
rat
Livello qualitativo
Forma dell’anticorpo
purified immunoglobulin
Tipo di anticorpo
primary antibodies
Clone
4H7, monoclonal
Reattività contro le specie
mouse
Reattività contro le specie (prevista in base all’omologia)
rat (based on 100% sequence homology), porcine (based on 100% sequence homology)
tecniche
ChIP: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Isotipo
IgG2aκ
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
wet ice
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
mouse ... Mecp2(17257)
Descrizione generale
DNA methyltransferases methylate the 5-position of cytosine in the context of CpG dinucleotides. DNA methylation is crucial for normal embryonic development, imprinting, and X chromosome inactivation. Methyl CpG binding proteins (MeCPs) specifically recognize methylated regions of DNA. It represses transcription both directly and by association with known corepressor proteins which include members of the histone deacetylase protein families.
Specificità
This antibody recognizes the C-terminus of MeCP2, which has been epitope mapped between amino acids 310-492.
Immunogeno
Epitope: C-terminus
Full length, strep-tagged N-terminus of rat MeCP2.
Applicazioni
Immunoprecipitation Analysis: A representative lot was used by an an independent laboratory to detect MeCP2 in various, mouse whole brain cell lysates (Jost, K. L., et al. (2011). PLoS ONE. 6(11):e26499).
Chromatin Immunoprecipitation Analysis: A representative lot was used by an an independent laboratory to detect MeCP2 in various, mouse WT, but not in MeCP2 knockout brain nuclear extracts (Jost, K. L., et al. (2011). PLoS ONE. 6(11):e26499).
Immunocytochemistry Analysis: A representative lot was used by an an independent laboratory to detect MeCP2 in various, GFP-meCP2 transfected C2C12 cells (Jost, K. L., et al. (2011). PLoS ONE. 6(11):e26499).
Immunohistochemistry Analysis: A representative lot was used by an an independent laboratory to detect MeCP2 in various, mouse WT, but not in MeCP2 hemizygous null brain tissue sections (Jost, K. L., et al. (2011). PLoS ONE. 6(11):e26499).
Chromatin Immunoprecipitation Analysis: A representative lot was used by an an independent laboratory to detect MeCP2 in various, mouse WT, but not in MeCP2 knockout brain nuclear extracts (Jost, K. L., et al. (2011). PLoS ONE. 6(11):e26499).
Immunocytochemistry Analysis: A representative lot was used by an an independent laboratory to detect MeCP2 in various, GFP-meCP2 transfected C2C12 cells (Jost, K. L., et al. (2011). PLoS ONE. 6(11):e26499).
Immunohistochemistry Analysis: A representative lot was used by an an independent laboratory to detect MeCP2 in various, mouse WT, but not in MeCP2 hemizygous null brain tissue sections (Jost, K. L., et al. (2011). PLoS ONE. 6(11):e26499).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Chromatin Biology
This MeCP2 antibody is validated for use in WB, IHC, ICC, ChIP & IP for the detection of the MeCP2 protein
Qualità
Evaluated by Western Blot in mouse brain tissue nuclear extract.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected MeCP2 in 10 µg of mouse brain tissue nuclear extract.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected MeCP2 in 10 µg of mouse brain tissue nuclear extract.
Descrizione del bersaglio
~75 kDa observed. Uniprot describes an isoform produced by alternative splicing at ~55 kDa The calculated molecular weight is 53 kDa, however MeCP2 has been observed between ~75-100 kDa in western blots (Jost, K. L., et al. (2011). PLoS ONE. 6(11):e26499).
Stato fisico
Format: Purified
Protein G Purified
Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Stoccaggio e stabilità
Stable for 1 year at 2-8°C from date of receipt.
Risultati analitici
Control
Mouse brain tissue nuclear extract
Mouse brain tissue nuclear extract
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Generation and characterization of rat and mouse monoclonal antibodies specific for MeCP2 and their use in X-inactivation studies.
Jost, KL; Rottach, A; Milden, M; Bertulat, B; Becker, A; Wolf, P; Sandoval, J; Petazzi et al.
Testing null
Camille Ravel-Godreuil et al.
iScience, 24(7), 102756-102756 (2021-07-20)
Age is a major risk factor for neurodegenerative diseases like Parkinson's disease, but few studies have explored the contribution of key hallmarks of aging, namely DNA methylation changes and heterochromatin destructuration, in the neurodegenerative process. Here, we investigated the consequences
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