MABD411

Anti-BMP4 Antibody, clone PA354-16.1.1

clone PA354-16.1.1, from mouse

• Sinonimo/i: Bone morphogenetic protein 4, BMP-4, Bone morphogenetic protein 2B, BMP-2B
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.75

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: PA354-16.1.1, monoclonal
• Reattività contro le specie: human
• tecniche: flow cytometry: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotipo: IgG2aκ
• N° accesso NCBI: NP_001193
• N° accesso UniProt: P12644
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... BMP4(652)

Descrizione

Descrizione generale

Bone morphogenetic protein 4 (BMP4), also known as Bone morphogenetic protein 4 (BMP-4), Bone morphogenetic protein 2B (BMP-2B), and encoded by the gene name BMP4/ BMP2B/ DVR4, is morphogenetic protein that induces cartilage and bone formation. Similar to other bone morphogenetic proteins, BMP4 has been shown to play an important role in bone and cartilage development, specifically tooth and limb development and fracture repair. It has been shown to be involved in muscle development, bone mineralization, and uteric bud development. BMP4 has also been implicated in Fibrodysplasia Ossificans Progressiva. In human embryonic development, BMP4 is a critical signalling molecule required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. BMP4 is secreted from the dorsal portion of the notochord, and it acts in concert with sonic hedgehog (released from the ventral portion of the notochord) to establish a dorsal-ventral axis for the differentiation of later structures. BMP4 stimulates differentiation of overlying ectodermal tissue. Inhibition of the BMP4 signal (by chordin, noggin, or follistatin) causes the ectoderm to differentiate into the neural plate. If these cells also receive signals from FGF, they will differentiate into the spinal cord; in the absence of FGF the cells become brain tissue.

Immunogeno

MBP-tagged recombinant protein corresponding to human BMP4.

Applicazioni

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected BMP4 in human pancreas tissue.
Flow Cytometry Analysis: 1:200 dilution of this antibody from a representative lot detected BMP4 in Caco-2 cells (Data courtesy of Juan Ignacio Imbaud, Protein Alternatives).

Research Category
Stem Cell Research

Research Sub Category
Cell Cycle, DNA Replication & Repair

This Anti-BMP4 antibody is validated for use in WB, FC, IH for the detection of BMP4.

Qualità

Evaluated by Western Blotting in human recombinant protein BMP4.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected 0.01 µg human recombinant protein BMP4.

Descrizione del bersaglio

47 kDa calculated. The calculated molecular weight is 47 kDa, however BMP4 has been shown as a ~80 and ~90 kDa band in western blots with the use of a MBP tag.

Stato fisico

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1