MAB5256X
Anti-Neurofilament H Antibody, clone NE14, Alexa Fluor™ 488 Conjugated
clone NE14, from mouse, ALEXA FLUOR™ 488
Sinonimo/i:
200 kDa neurofilament protein, Neurofilament triplet H protein, neurofilament, heavy polypeptide, neurofilament, heavy polypeptide 200kDa
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
Coniugato
ALEXA FLUOR™ 488
Forma dell’anticorpo
purified immunoglobulin
Tipo di anticorpo
primary antibodies
Clone
NE14, monoclonal
Reattività contro le specie
human, rat, pig, porcine
tecniche
immunocytochemistry: suitable
immunohistochemistry: suitable
Isotipo
IgG1
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
wet ice
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... NEFH(4744)
pig ... Nefh(100156492)
rat ... Nefh(24587)
Descrizione generale
Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexes of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure; however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments.
Specificità
Reactivity with other species has not been tested.
This antibody recognizes Neurofilament H.
Immunogeno
Epitope: Unknown
Purified neurofilament polypeptides (Debus et al., 1983).
Applicazioni
Anti-Neurofilament H Antibody, clone NE14, Alexa Fluor 488 Conjugated is an antibody against Neurofilament H for use in IH & IC.
Immunohistochemistry:
5-10 µg/mL on frozen brain tissue. Optimal working dilutions must be determined by end user.
5-10 µg/mL on frozen brain tissue. Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurofilament & Neuron Metabolism
Neurofilament & Neuron Metabolism
Qualità
Immunocytochemical Analysis: 1:250 dilution of this lot detected Neurofilament-H in rat cortex cell culture.
Descrizione del bersaglio
~ 200 kDa
Stato fisico
Protein G Purified
Purified immunoglobulin in 0.02M Phosphate buffer, 0.25M NaCl containing 0.1% sodium azide.
Stoccaggio e stabilità
Stable for 6 months at 2-8ºC from date of receipt.
Risultati analitici
Control
Rat Cortex Cells.
Rat Cortex Cells.
Altre note
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Note legali
ALEXA FLUOR is a trademark of Life Technologies
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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