MAB374-AF488

Anti-GAPDH Antibody, clone 6C5, Alexa Fluor^™ 488 conjugate

clone 6C5, from mouse, ALEXA FLUOR^™ 488

• Sinonimo/i: Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH, GAPDH
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Coniugato: ALEXA FLUOR^™ 488
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: 6C5, monoclonal
• Reattività contro le specie: mouse, human
• Reattività contro le specie (prevista in base all’omologia): cat (based on 100% sequence homology), fish (based on 100% sequence homology), canine (based on 100% sequence homology), porcine (based on 100% sequence homology), rat (based on 100% sequence homology), rabbit (based on 100% sequence homology)
• tecniche: immunocytochemistry: suitable
• Isotipo: IgG1
• N° accesso NCBI: NM_001082253.1
• N° accesso UniProt: P46406
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... GAPDH(2597) ; mouse ... Gapdh(14433) ; rabbit ... Gapdh(100009074)

Descrizione

Descrizione generale

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an ubiquitous glycolytic enzyme present in reasonably high levels in almost all tissues. The complete and functional enzyme is a tetramer with each of four identical subunits occupying the vertex of a tetrahedron. Its binding domains also include one principally interacting with NAD+ and another interacting with glyceraldehyde 3-phosphate (GAP). As a ′house-keeping′ enzyme, it catalyzes the synthesis of 1,3-biphosphoglycerate, a "high energy" intermediate used for the synthesis of ATP. Besides its cytoplasmic role in metabolism, it is also involved in the initial stages of apoptosis or oxidative stress response where it is translocated to the nucleus. Such actions may reflect the role of GAPDH in DNA repair or as one nuclear carrier for apoptotic molecules. GAPDH can also bind specifically to proteins implicated in the pathogenesis of a variety of neurodegenerative disorders, including the beta-amyloid precursor protein and the Huntingtin protein where reduced function of GAPDH is associated with Alzheimer′s and Huntington′s disease fibroblasts. GAPDH has also been identified as a potential target for nitric oxide (NO)-mediated cellular toxicity.

Specificità

It has been reported that this antibody does not react with GAPDH from E. coli.

Applicazioni

This Anti-GAPDH Antibody, clone 6C5, Alexa Fluor^™ 488 conjugate is validated for use in Immunocytochemistry for the detection of GAPDH.

Qualità

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected GAPDH in A431 cells.

Descrizione del bersaglio

~38 kDa observed

Stato fisico

Purified mouse conjugate antibody in buffer containing PBS 15 mg/ml BSA and 0.1% Sodium Azide.

Altre note

Concentration: Please refer to lot specific datasheet.

Note legali

ALEXA FLUOR is a trademark of Life Technologies

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable