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MAB3317

Sigma-Aldrich

Anti-MMP-14 Antibody, hemopexin domain, clone 113-5B7

clone 113-5B7, Chemicon®, from mouse

Sinonimo/i:

MT1-MMP

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
113-5B7, monoclonal
application:
ELISA
IHC
WB
Reattività contro le specie:
rat, human, bovine, mouse
tecniche:
ELISA: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
citations:
17

Origine biologica

mouse

Livello qualitativo

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

113-5B7, monoclonal

Reattività contro le specie

rat, human, bovine, mouse

Produttore/marchio commerciale

Chemicon®

tecniche

ELISA: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

Isotipo

IgG3κ

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

dry ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... MMP14(4323)

Specificità

Reacts with human MMP-14, also known as MT1-MMP, with a very slight cross-reactivity to hMMP-3. For unknown reasons this antibody does not react on westerns to CC1043.

Immunogeno

CDGNFDTVAMLRGEM in the hemopexin-like domain of human MT1-MMP
Epitope: a.a. 319-333

Applicazioni

Anti-MMP-14 Antibody, hemopexin domain, clone 113-5B7 is an antibody against MMP-14 for use in ELISA, IH(P) & WB.
Research Category
Cell Structure
Research Sub Category
MMPs & TIMPs
Western Blot: 10 μg/mL, membrane preparations; MD-MB-231 cells pretreated with 1-5 μg/mL ConA for 24-48 hours as positive control. 60-65 kDa proteins are identified.


When tested against recombinant MT1-MMP (lacking the TM domain) expressed in CHO cells the antibody reacts with a band of ~59 kDa.


Immunohistochemistry: Frozen tissue sections; traditional formalin fixation is problematic. Paraffin-embedded tissue sections with PLP fixation required.


Immunoprecipitation


EIA


Optimal working dilutions must be determined by the end user.

Descrizione del bersaglio

~59-69 kDa

Stato fisico

Format: Purified
Protein A purified
Purified immunoglobulin. Liquid in 0.1 M sodium phosphate buffer, pH 7.0, containing 2% protease-free bovine Serum albumin.

Stoccaggio e stabilità

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Risultati analitici

Control
Breast carcinoma tissue

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Manufactured by Daiichi Fine Chemical Co., Ltd

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Raccomandato

N° Catalogo
Descrizione
Determinazione del prezzo

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Dynamic expression of matrix metalloproteinases (MMP-2, -9 and -14) and the tissue inhibitors of MMPs (TIMP-1, -2 and -3) at the implantation site during tubal pregnancy.
Bai, SX; Wang, YL; Qin, L; Xiao, ZJ; Herva, R; Piao, YS
Reproduction (Cambridge, England) null
Vasculostatin inhibits intracranial glioma growth and negatively regulates in vivo angiogenesis through a CD36-dependent mechanism.
Kaur, B; Cork, SM; Sandberg, EM; Devi, NS; Zhang, Z; Klenotic, PA; Febbraio, M; Shim et al.
Cancer Research null
White matter microglia produce membrane-type matrix metalloprotease, an activator of gelatinase A, in human brain tissues.
Yamada, T, et al.
Acta neuropathologica, 90, 421-424 (1995)
Carla S Ceron et al.
Matrix biology : journal of the International Society for Matrix Biology, 31(4), 261-270 (2012-02-22)
Increased vascular matrix metalloproteinases (MMPs) levels play a role in late phases of hypertensive vascular remodeling. However, no previous study has examined the time course of MMPs in the various phases of two-kidney, one-clip hypertension (2K1C). We examined structural vascular
Amit Ranjan et al.
BioMed research international, 2014, 804680-804680 (2014-09-03)
Matrix remodeling and invasion of basement membrane are the major determinants of malignant progression. Matrix degrading enzymes play a pivotal role in this process and have been shown to be regulated at multiple levels. Using high metastatic B16F10 and its

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