MAB3222
Anti-BrdU Antibody, clone IIB5
clone IIB5, Chemicon®, from mouse
Sinonimo/i:
BrdU
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
Forma dell’anticorpo
purified immunoglobulin
Tipo di anticorpo
primary antibodies
Clone
IIB5, monoclonal
Reattività contro le specie (prevista in base all’omologia)
all
Produttore/marchio commerciale
Chemicon®
tecniche
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
Isotipo
IgG1
Condizioni di spedizione
wet ice
modifica post-traduzionali bersaglio
unmodified
Descrizione generale
The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
Specificità
Reacts specifically with bromodeoxyuridine. It also recognizes BrdU when incorporated into nuclear DNA.
Immunogeno
A fusion of mouse myeloma cells with spleen cells from a mouse intraperitoneally immunized with BrdU conjugated to bovine serum albumin.
Applicazioni
Anti-BrdU Antibody, clone IIB5 is a Mouse Monoclonal Antibody for detection of Bromodeoxyuridine also known as BrdU & has been validated in FC, ICC, IHC(P).
Immunohistochemistry: 1:100-1:200 on frozen and paraffin embedded tissues with acid/proteinase treatments. See:
http://www.protocol-online.org/prot/Immunology/Immunohistochemistry. For examples of various protocols.
Flow Cytometry: 1:100-1:200; acid methanol treatment required.
Optimal working dilutions must be determined by the end user.
In order for the antibody to work a pretreatment to expose DNA will be needed (1 mg/mL of pepsin in 0.01 M HCL 30 min at r/t)
http://www.protocol-online.org/prot/Immunology/Immunohistochemistry. For examples of various protocols.
Flow Cytometry: 1:100-1:200; acid methanol treatment required.
Optimal working dilutions must be determined by the end user.
In order for the antibody to work a pretreatment to expose DNA will be needed (1 mg/mL of pepsin in 0.01 M HCL 30 min at r/t)
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Stato fisico
Format: Purified
Protein A Purified mouse immunoglobulin in PBS with 0.09% sodium azide.
Protein A purified
Stoccaggio e stabilità
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Risultati analitici
Control
After incorporation of BrdU, all DNA containing species
After incorporation of BrdU, all DNA containing species
Altre note
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Note legali
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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