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Key Documents

MAB1680

Sigma-Aldrich

Anti-Filamin A Antibody, clone TI10

ascites fluid, clone TI10, Chemicon®

Sinonimo/i:

Alpha-Filamin, Filamin I, Endothelial Actin-binding Protein, ABP-280, Nonmuscle Filamin

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

Forma dell’anticorpo

ascites fluid

Tipo di anticorpo

primary antibodies

Clone

TI10, monoclonal

Reattività contro le specie

human, bovine

Non deve reagire con

rat, mouse

Produttore/marchio commerciale

Chemicon®

tecniche

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable

Isotipo

IgG1

N° accesso UniProt

Condizioni di spedizione

dry ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

mouse ... Flna(192176)

Descrizione generale

Filamin is a structural protein that forms flexible cross-links between two actin filaments. Filamin is a homodimer of polypeptide chains each joined to the other at one end with an actin binding site ath the other. It is present in smooth muscle, fibroblasts, platelets and lymphocytes.

Specificità

Human filamin (actin-bidning protein). Recognizes unprocessed (270-280 kDa) and the C-terminal 90 kDa calpain cleavage fragment of filamin (Aakhus, 1992).

Immunogeno

Human platelet protein

Applicazioni

Detect Filamin A using this Anti-Filamin A Antibody, clone TI10 validated for use in IP, WB, IH, IH(P).
Immunoblotting: 1:250 to 1:1000

Immunoprecipitation:Suggested lysis buffer is PBS with 0.5% triton X-100 with proteinase inhibitors (note for full length filamin include calpain inhibitors). 5 microliters of antibody for every 300μL of cell lysate (3-5mg/ml total protein is suggested). Incubation is 4 hours RT or overnight 4C; Protein A/G agarose beads or rabbit anti-mouse secondary capture antibody is recommended for best recovery. 4-10% acrylamide gels are recommended for full length filamin or the 80kDa fragement visualization.

Immunofluorescence: 1:50 to 1:200 using standard ABC technique. Suitable for staining of paraffin embedded sections (lower dilutions). High temperature citrate buffer antigen retrieval technique recommended.

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Cytoskeleton

Linkage

Replaces: CBL229

Stato fisico

Ascites. Liquid

Stoccaggio e stabilità

Maintain frozen at -20°C for up to 12 months in undiluted aliquots. Avoid repeated freeze/thaw cycles.

Risultati analitici

Control
Positive control tisse: skin.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Raccomandato

N° Catalogo
Descrizione
Determinazione del prezzo

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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A 90 kDa fragment of filamin A promotes Casodex-induced growth inhibition in Casodex-resistant androgen receptor positive C4-2 prostate cancer cells.
Wang, Y; Kreisberg, JI; Bedolla, RG; Mikhailova, M; deVere White, RW; Ghosh, PM
Oncogene null
Identification of candidate prostate cancer biomarkers in prostate needle biopsy specimens using proteomic analysis.
Jian-Feng Lin,Jun Xu,Hong-Yu Tian,Xia Gao,Qing-Xi Chen,Qi Gu,Gen-Jun Xu,Jian-da Song,Fu-Kun Zhao
International Journal of Cancer. Journal International Du Cancer null
Kun Wang et al.
Molecular medicine reports, 20(4), 3671-3678 (2019-09-06)
The metastasis and recurrence rate, and the overall prognosis of colorectal cancer (CRC) remain unsatisfactory. Filamin A (FLNa), as an actin‑binding protein, can interact with various signaling molecules and membrane receptors to affect cell signal transduction and function. However, whether
Structure of three tandem filamin domains reveals auto-inhibition of ligand binding.
Yatish Lad,Tiila Kiema,Pengju Jiang,Olli T Pentikainen,Charlotte H Coles,Iain D Campbell et al.
The Embo Journal null
Enhancing the effectiveness of androgen deprivation in prostate cancer by inducing Filamin A nuclear localization.
Mooso, Benjamin A, et al.
Endocrine-Related Cancer, 19, 759-777 (2012)

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