MAB1621

Anti-Neurofilament 145 kDa Antibody, CT, clone 3H11

clone 3H11, Chemicon^®, from mouse

• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: 3H11, monoclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: avian
• Reattività contro le specie (prevista in base all’omologia): mammals
• Produttore/marchio commerciale: Chemicon^®
• tecniche: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotipo: IgG1
• N° accesso NCBI: NM_001105541.1; NM_005382.2
• N° accesso UniProt: P07197
• Condizioni di spedizione: dry ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... NEFM(4741)

Descrizione

Descrizione generale

Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information see Nervous System Cell Type Specific Marker chart online under the CHEMICON Technical Support section.

Specificità

Reacts with an epitope dependent on the last 84 amino acids of rat NF-M (145K). Reacts specifically with NF-M from a variety of mammalian and avian species. The antibody is not sensitive to the phosphorylation state of the neurofilament.

Immunogeno

Recombinant fusion protein containing the last 168 amino acids of rat NF-M. Purified from E. Coli inclusion bodies by ion exchange chromatography on DEAE-cellulose in 6 M urea.

Applicazioni

Anti-Neurofilament 145 kDa Antibody, C-terminus, clone 3H11 detects level of Neurofilament 145 kDa & has been published & validated for use in WB, IC, IH.

Immunohistochemistry: 1:50-1:1000; fresh frozen, 4% PFA fixed tissue sections

Immunoblotting: 1:1000 - 1:2000 ECL detection.

Optimal working dilutions must be determined by end user.

Stato fisico

Affinity purified antibody in PBS, 50% glycerol, and 5 mM sodium azide

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable