IM27L
Anti-Cathepsin B (Ab-1) Mouse mAb (CA10)
lyophilized, clone CA10, Calbiochem®
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About This Item
Codice UNSPSC:
12352203
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
Forma dell’anticorpo
purified antibody
Tipo di anticorpo
primary antibodies
Clone
CA10, monoclonal
Forma fisica
lyophilized
non contiene
preservative
Reattività contro le specie
porcine, human, bovine
Non deve reagire con
rat
Produttore/marchio commerciale
Calbiochem®
Condizioni di stoccaggio
OK to freeze
Isotipo
IgG2a
Condizioni di spedizione
ambient
Temperatura di conservazione
2-8°C
Informazioni sul gene
mouse ... Ctsb(13030)
Descrizione generale
Anti-Cathepsin B (Ab-1), mouse monoclonal, clone CA10, recognizes the ~43 kDa latent and ~31 kDa active forms of cathepsin B in HepG2 cells. It is validated for WB, IF, and IP.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/O-NS1 plasmacytoma cells. Recognizes the ~43 kDa latent and the ~31 kDa active forms of cathepsin B.
Recognizes the ~43 kDa latent and the ~31 kDa active forms of cathepsin B in HepG2 cells.
Immunogeno
Epitope: within the heavy chain of mature cathepsin B
Human
purified human liver cathepsin B
Applicazioni
Immunoblotting (250 ng/ml)
Immunoprecipitation (1 µg/ml)
Immunofluorescence (see comments)
Immunoprecipitation (1 µg/ml)
Immunofluorescence (see comments)
Attenzione
Toxicity: Standard Handling (A)
Stato fisico
Lyophilized from 20 mM ammonium bicabonate buffer, 100 µg BSA (100 µg size) or 20 µg BSA (20 µg size).
Ricostituzione
Resuspend the lyophilized antibody with 1 ml sterile PBS. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Store at 4°C until reconstituted, then store in aliquots at -20°C or at 4°C with 0.1% azide. Freezing of aliquots is best for long-term storage of reconstituted product. Avoid freeze/thaw cycles.
Risultati analitici
Positive Control
Hep G2 cells
Hep G2 cells
Altre note
Sloane, B.F. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1:137-152.
Barret, A.J. 1987. Trends Biochem. Sci.12, 193.
Chan, S.J., et al. 1986. Proc. Natl. Acad. Sci. USA83, 7721.
Barrett, A.J. and Kirschke, H. 1981. Meth. Enzymol.80, 535.
Barret, A.J. 1987. Trends Biochem. Sci.12, 193.
Chan, S.J., et al. 1986. Proc. Natl. Acad. Sci. USA83, 7721.
Barrett, A.J. and Kirschke, H. 1981. Meth. Enzymol.80, 535.
This antibody recognizes an epitope on the heavy chain of mature cathepsin B and therefore detects both mature and pro-cathepsin B. Also reported to work for immunofluorescence. Antibody should be titrated for optimal results in individual systems.
Note legali
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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