HTS100RTA
Ready-to-Assay delta Opioid Receptor Frozen Cells
Human
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About This Item
Codice UNSPSC:
41106514
eCl@ss:
32011203
NACRES:
NA.81
Prodotti consigliati
product name
Ready-to-Assay delta Opioid Receptor Frozen Cells, Human Delta / OP1 / DOP / DOR GPCR frozen cells for Calcium Flux FLIPR Assays.
Origine biologica
human
Livello qualitativo
Produttore/marchio commerciale
Ready-to-Assay
tecniche
calcium flux assay: suitable
N° accesso NCBI
Metodo di rivelazione
fluorometric
Condizioni di spedizione
dry ice
Descrizione generale
Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.
Opiates derived from the opium poppy, Papaver somniferum, have been used in for millenia for their anti-diarrheal, analgesic and euphoric properties. More recently, endogenous peptides, enkephalins, dynorphins, and endorphins, were found to bind to the same sites as opiate alkaloids. The receptors for the classical opioids are three related GPCRs, δ, κ, andμ also known as OP1, OP2 and OP3, respectively), that activate Gi/o to reduce intracellular cAMP levels. Although most clinically used opioids function by activation of the μ opioid receptor, agonist of spinal δ opioid receptors have antinociceptive activity that is independent of μ. In addition, activation of δ increases locomotor activity, inhibits gastrointestinal motility, and decreases respiratory frequency (Dhawan et al., 1996). Agonists for δ opioid receptors also exhibit antidepressant-like activity in animal models (Broom et al., 2002). Millipore’s cloned human δ opioid -expressing cell line is made in the Chem-1 host, which supports high levels of recombinant δ opioid expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at δ.
Opiates derived from the opium poppy, Papaver somniferum, have been used in for millenia for their anti-diarrheal, analgesic and euphoric properties. More recently, endogenous peptides, enkephalins, dynorphins, and endorphins, were found to bind to the same sites as opiate alkaloids. The receptors for the classical opioids are three related GPCRs, δ, κ, andμ also known as OP1, OP2 and OP3, respectively), that activate Gi/o to reduce intracellular cAMP levels. Although most clinically used opioids function by activation of the μ opioid receptor, agonist of spinal δ opioid receptors have antinociceptive activity that is independent of μ. In addition, activation of δ increases locomotor activity, inhibits gastrointestinal motility, and decreases respiratory frequency (Dhawan et al., 1996). Agonists for δ opioid receptors also exhibit antidepressant-like activity in animal models (Broom et al., 2002). Millipore’s cloned human δ opioid -expressing cell line is made in the Chem-1 host, which supports high levels of recombinant δ opioid expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at δ.
Descrizione della linea cellulare
GPCR Cell Lines
Host cells: Chem-1
Azioni biochim/fisiol
GPCR Class: A
Protein Target: Delta / OP1 / DOP / DOR
Target Sub-Family: Opioid
Componenti
Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
Fifty (50) mL of Media Component.
Fifty (50) mL of Media Component.
Altre note
Limited to a single use and shall not be propagated and/or re-frozen by licensee.
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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