CP53

Anti-ICAM-1 (Ab-2) Mouse mAb (W-CAM-1)

liquid, clone W-CAM-1, Calbiochem^®

• Sinonimo/i: Anti-CD54, Anti-Inter-Cellular Adhesion Molecule, Anti-Inter-Cellular Adhesion Molecule, Anti-CD54
• Codice UNSPSC: 12352203

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: W-CAM-1, monoclonal
• Forma fisica: liquid
• contiene: ≤0.1% sodium azide as preservative
• Reattività contro le specie: human
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze
• Isotipo: IgG2b
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: 2-8°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... ICAM1(3383)

Descrizione

Descrizione generale

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing Balb/c splenocytes with p3-NS-1/Ag4-1 (NS1) mouse myeloma cells (see application references). Recognizes the ~85-115 kDa ICAM-1 protein.

Recognizes the ~85-115 kDa ICAM-1 protein in endothelial cells and tonsil tissue.

This Anti-ICAM-1 (Ab-2) Mouse mAb (W-CAM-1) is validated for use in Cell Inhibition Assays, FC, Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of ICAM-1 (Ab-2).

Immunogeno

Human

Raji cells

Applicazioni

Cell Inhibition Assays (see comments)

Flow Cytometry (see comments)

Frozen Sections (1:50-1:100)

Immunoblotting (1:200-1:400)

Immunofluorescence (see comments)

Immunoprecipitation (10 µl/mg protein lysate)

Paraffin Sections (not recommended)

Confezionamento

Please refer to vial label for lot-specific concentration.

Attenzione

Toxicity: Standard Handling (A)

Stato fisico

In 10 mM PBS, 0.2% BSA, pH 7.4.

Ricostituzione

Following initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.

Risultati analitici

Positive Control
Endothelial cells or tonsil tissue

Altre note

Takami, S., et al. 1998. Circ.97, 721.
Craig, A.G., et al. 1997. Infect. Immun.65, 4580.
DeGraba, T.J. 1997. Neurology49, S15.
Elangbam, C.S., et al. 1997. Vet. Pathol. 34, 61.
McCormick, C.J., et al. 1997. J. Clin. Invest.15, 2521.
Acevedo, A., et al. 1993. Am. J. Pathol.143, 774.
De Fougerolles, A.R., et al. 1993. J. Exp. Med.177, 1187.
Fawcett, J., et al. 1992. Nature360, 481.
Vazeux, R. et al. 1992. Nature360, 485.
Boyd, A.W., et al. 1989. Blood73, 1896.

This antibody will block aggregation of cell lines mediated by ICAM-1 and blocks homotypic binding of purified populations of activated T- and B-lymphocytes and aggregation of mixed T- and B-cell blasts. Will inhibit T-cell adhesion to normal human endothelial cells. Activation induced by cell-cell contact (mixed lymphocyte reaction, T cell mediated B-cell activation) is significantly inhibited. This antibody will also block elements of both effector arms of the immune system (cytotoxic cell function and Ig production). This antibody has been reported to work for immunofluorescence and flow cytometry studies. Antibody should be titrated for optimal results in individual systems.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable