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Key Documents

CBL555

Sigma-Aldrich

Anti-CD34 Class III Antibody, clone 581

clone 581, Chemicon®, from mouse

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

Forma dell’anticorpo

affinity isolated antibody

Tipo di anticorpo

primary antibodies

Clone

581, monoclonal

Reattività contro le specie

human

Produttore/marchio commerciale

Chemicon®

tecniche

flow cytometry: suitable

Isotipo

IgG1

N° accesso UniProt

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... CD34(947)

Categorie correlate

Specificità

Clone 581 reacts with the class III CD34 epitope is resistant to neuraminidase, chymopapain and glycoprotease.

The antibody reacts with a single-chain 105-120 kDa heavily O-glycosylated transmembrane glycoprotein which is expressed on haematopoietic progenitor cells, vascular endothelium and some tissue fibroblasts. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C suggesting a putative role in signal transduction. CD34 may also play a role in the adhesion of specific antigens to endothelium.

Applicazioni

Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
Study of the Class III CD34 epitope by FACS

Optimal working dilutions must be determined by the end user.
This Anti-CD34 Antibody, Class III, clone 581 is validated for use in FC for the detection of CD34 Class III.

Stato fisico

Format: Purified
Protein G affinity chromatography. The monoclonal is presented at a concentration of 100μg/1ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

Stoccaggio e stabilità

For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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Positive selection of bone marrow-derived CD34 positive cells for possible stem cell transplantation.
Egeland, T, et al.
Transplantation Proceedings, 25, 1261-1263 (1993)
Anthony Zulli et al.
Experimental physiology, 93(5), 564-569 (2008-01-15)
Angiotensin-converting enzyme 2 (ACE2) is a novel enzyme with possible implications in the treatment of blood pressure disorders. Recent evidence suggests that an upregulation of ACE2 can be stimulated by all-trans retinoic acid (at-RA); however, at-RA also affects regulation of
Teresa Massam-Wu et al.
Methods in molecular biology (Clifton, N.J.), 1722, 249-260 (2017-12-22)
We present a method to capture mesenchymal stromal cells (MSCs) by adhesion to extracellular matrix (ECM) molecules under flow conditions. The technique simulates a physiological system and exploits the natural biological interactions of cells, through integrin receptors, with their ECM.
Mahmoud Yousefifard et al.
Journal of stem cells & regenerative medicine, 18(2), 53-63 (2023-01-31)
Few studies are conducted on the efficacy of human adipose-derived stem cells (ADSCs) in spinal cord injury (SCI) management and electrophysiological changes in the spinal cord. Therefore, the present study aimed to determine the effect of ADSCs on neuropathic pain

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