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Key Documents

CBL218

Sigma-Aldrich

Anti-Keratin K3/K76 Antibody, clone AE5

clone AE5, Chemicon®, from mouse

Sinonimo/i:

Anti-HUMCYT2A, Anti-KRT2P

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

Forma dell’anticorpo

purified antibody

Tipo di anticorpo

primary antibodies

Clone

AE5, monoclonal

Reattività contro le specie

human, mouse, bovine, rabbit

Produttore/marchio commerciale

Chemicon®

tecniche

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

Isotipo

IgG1

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... KRT3(3850)

Specificità

The antibody reacts with the 64 kDa polypeptide (cytokeratin 3; Keratin K3) of corneal epithelium and keratin K76 (cytokeratin K2p) of palate epithelium. As such this represents an excellent marker for studying corneal epithelial-type differentiation. Also recognizes bovine snout and lip epithelium, as well as a rabbit corneal cell line.

Immunogeno

Rabbit corneal epithelial keratin

Applicazioni

Anti-Keratin K3/K76 Antibody, clone AE5 is an antibody against Keratin K3/K76 for use in WB, IH, IH(P).
Immunohistochemistry on frozen sections: 1:50.

Immunohistochemistry on Paraffin-embedded sections: 1:50 on paraffin-embedded tissue sections fixed in 10% neutral buffered formalin or Fekete’s acid-alcohol-formalin following trypsin digestion antigen retrieval (Mikaelian et al., 2004).

Western blotting: 1:500.

Optimal working dilutions must be determined by the end user.

Descrizione del bersaglio

64 kDa

Stato fisico

Format: Purified
The monoclonal was purified by ion exchange chromatography is presented in phosphate buffered saline (pH 7.2) containing 0.09% sodium azide.

Stoccaggio e stabilità

For use within 1 month of purchase store at 2°-8°C, for long term storage aliquot antibody into small volumes and store at -20°C for up to one year.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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