CBL208
Anti-Cytokeratin 20 Antibody, clone IT-Ks20.8
clone IT-Ks20.8, Chemicon®, from mouse
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
Forma dell’anticorpo
purified immunoglobulin
Tipo di anticorpo
primary antibodies
Clone
IT-Ks20.8, monoclonal
Reattività contro le specie
human
Non deve reagire con
mouse, rat
Produttore/marchio commerciale
Chemicon®
tecniche
immunohistochemistry: suitable (paraffin)
western blot: suitable
Isotipo
IgG2a
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
wet ice
Informazioni sul gene
human ... KRT20(54474)
mouse ... Krt20(66809)
rat ... Krt20(286912)
Descrizione generale
IT-Ks 20.8 represents an excellent marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma). Adenocarcinomas of breast, lung, endometrium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially negative.
Specificità
The antibody is specific for the 46 kDa polypeptide (cytokeratin 20).
Immunogeno
Electrophoretically purified cytokeratin 20 from human intestinal mucosa
Applicazioni
Western blotting
Immunohistochemistry: 1:10 dilution of stock on frozen tissues, with one hour incubation at room temp or overnight at 2°-8°C. For paraffin sections, 1:100 dilution of antibody with protease pretreatment and/or heat induced epitope retrieval recommended.
Cytological material
Studies of cultured cell lines HT-29, LoVo, DLD-1, SW1116, CaCo-2 & RT-4.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry: 1:10 dilution of stock on frozen tissues, with one hour incubation at room temp or overnight at 2°-8°C. For paraffin sections, 1:100 dilution of antibody with protease pretreatment and/or heat induced epitope retrieval recommended.
Cytological material
Studies of cultured cell lines HT-29, LoVo, DLD-1, SW1116, CaCo-2 & RT-4.
Optimal working dilutions must be determined by the end user.
Detect Cytokeratin 20 using this Anti-Cytokeratin 20 Antibody, clone IT-Ks20.8 validated for use in WB, IH(P).
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytokeratins
Cytokeratins
Linkage
Replaces: MABT150
Stato fisico
Format: Purified
Protein A purified immunoglobulin lyophilized from PBS, pH 7.4, containing 0.5% BSA and 0.09% sodium azide. Reconstitute with 1 mL distilled water.
Stoccaggio e stabilità
Once reconstituted, maintain at 2°-8°C for up to 12 months from date of receipt. For extended storage, aliquots can be stored at -20°C. Avoid repeated freeze/thaw cycles.
Risultati analitici
Control
Positive cell lines include HT-29, LoVo, DLD-1, SW 1116, CaCo-2 and RT-4.
Positive cell lines include HT-29, LoVo, DLD-1, SW 1116, CaCo-2 and RT-4.
Note legali
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 3
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
P Harnden et al.
British journal of urology, 78(6), 870-875 (1996-12-01)
To investigate the dysregulation of cytokeratin 20 (CK20) expression in urothelial dysplasia and its potential as a diagnostic aid. Twenty-two patients were selected on the basis that they had undergone one or more biopsies showing dysplasia before the development of
V J Möbus et al.
British journal of cancer, 69(3), 422-428 (1994-03-01)
Two human ovarian (OV-MZ-10, OV-MZ-15) and two colon cancer cell lines (CO-MZ-5, CO-MZ-6) were newly established in permanent cell culture. These cell lines have been maintained in vitro for 5-6 years, the passage number varying from 25 to 228. They
The distribution and origin of keratin 20-containing taste buds in rat and human.
Zhang, C and Oakley, B
Differentiation, 61, 121-127 (1996)
Epithelial markers and differentiation in adnexal neoplasms of the skin: an immunohistochemical study including individual cytokeratins.
Demirkesen, C, et al.
Journal of cutaneous pathology, 22, 518-535 (1995)
I Moll et al.
Archives of dermatological research, 283(5), 300-309 (1991-01-01)
Human eccrine sweat gland ducts and benign and malignant eccrine poromas were studied for the expression of various cytokeratins (CK) and vimentin by applying immunoperoxidase and immunofluorescence microscopy to frozen or paraffin-embedded sections, and using two-dimensional gel electrophoresis and immunoblotting.
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