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Key Documents

AP192R

Sigma-Aldrich

Donkey Anti-Mouse IgG Antibody, Rhodamine conjugate, Species Adsorbed

Chemicon®, from donkey

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.46

Origine biologica

donkey

Livello qualitativo

Coniugato

TRITC conjugate
rhodamine conjugate

Forma dell’anticorpo

affinity purified immunoglobulin

Tipo di anticorpo

secondary antibodies

Clone

polyclonal

Reattività contro le specie

mouse

Produttore/marchio commerciale

Chemicon®

tecniche

immunofluorescence: suitable

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Descrizione generale

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Specificità

Mouse IgG

WAVELENGTH:

Absorption peak = 550 nm, Emission peak = 570 nm.

Applicazioni

Donkey anti-Mouse IgG Antibody, Rhodamine conjugate, Species Adsorbed is an antibody against Donkey Mouse IgG for use in IF.
Research Category
Secondary & Control Antibodies
Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling
Suggested dilution for most applications: 1:50-1:200

Optimal working dilutions must be determined by end user.

Stato fisico

Lyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA, and 0.05% sodium azide.

RECONSTITUTION:

Reconstitute with 500 μL of sterile distilled water.

RHODAMINE/PROTEIN:

Approximately 0.40 (A550/A280).

Stoccaggio e stabilità

Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Indicazioni di pericolo

Consigli di prudenza

Classi di pericolo

Aquatic Chronic 3

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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