ABT98

Anti-Kinectin Antibody

from rabbit, purified by affinity chromatography

• Sinonimo/i: Kinectin, CG-1 antigen, Kinesin receptor
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• Clone: polyclonal
• application: ICC; WB
• Reattività contro le specie: human
• tecniche: immunocytochemistry: suitable; western blot: suitable

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: human
• Reattività contro le specie (prevista in base all’omologia): rhesus macaque (based on 100% sequence homology)
• tecniche: immunocytochemistry: suitable; western blot: suitable
• N° accesso NCBI: NP_001072989
• N° accesso UniProt: Q86UP2
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... KTN1(3895)

Descrizione

Descrizione generale

Organelle transport is facilitated by molecular motors, such as kinesin and dynein, which interact with microtubules to transport cargo in an ATP-dependent manner. The shuttling of organelles is also dependent on kinectin, a transmembrane ER protein that associates with kinesin and stimulates its ATPase activity. Kinesin-kinectin dynamics have also been linked to focal adhesion growth. In addition, kinectin has been shown to activate the RhoG GTPase pathway which involves Rac1 and Cdc42 signaling. Kinectin is expressed in testis, ovary, spleen, thymus, prostate, colon, and small intestine.

Specificità

Other homologies: Mouse (82% Mouse sequence homology) and Rat (79% sequence homology).

This antibody recognizes the lumenal region of Kinectin.

Immunogeno

Epitope: Lumenal region

KLH-conjugated linear peptide corresponding to the lumenal region of human Kinectin.

Applicazioni

Detect Kinectin using this Anti-Kinectin Antibody validated for use in Western Blotting, ICC.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Kinectin in HeLa and A431 cells.

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

Qualità

Evaluated by Western Blot in human testis tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Kinectin in 10 µg of human testis tissue lysate.

Descrizione del bersaglio

~158 kDa observed. Uniprot describes 3 isoforms at ~149, ~150, and ~156 kDa

Stato fisico

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Risultati analitici

Control
Human testis tissue lysate

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable