ABS194

Anti-phospho PDHE1-A type I (Ser300) Antibody

from rabbit, purified by affinity chromatography

• Sinonimo/i: Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial, PDHE1-A type I
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: human
• Reattività contro le specie (prevista in base all’omologia): mouse (based on 100% sequence homology), rat (based on 100% sequence homology), bovine (based on 100% sequence homology), Xenopus (based on 100% sequence homology), zebrafish (based on 100% sequence homology)
• tecniche: immunocytochemistry: suitable; western blot: suitable
• N° accesso NCBI: NP_000275
• N° accesso UniProt: P08559
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: phosphorylation (pSer300)
• Informazioni sul gene: human ... PDHA1(5160)

Descrizione

Descrizione generale

In many organisms, the pyruvate dehydrogenase complex catalyzes the overall, irreversible conversion of pyruvate to acetyl-CoA and CO2 in the aerobic, energy-generating pathways, in addition to serving as a key regulator for cardiac substrate selection. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). PDH is regulated by both pyruvate dehydrogenase kinase (PDK)-mediated phosphorylation and feedback inhibition.

Specificità

This antibody recognizes PDHE1-A type I phosphorylated at Ser300.

Immunogeno

Epitope: Phosphorylated Ser300

KLH-conjugated linear peptide corresponding to human PDHE1-A type I phosphorylated at Ser300.

Applicazioni

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected PDHE1-A type I in dichloroacetate untreated and treated HEK 293 cells.

This Anti-phospho PDHE1-A type I (Ser300) Antibody is validated for use in WB, IC for the detection of phospho PDHE1-A type I (Ser300).

Qualità

Evaluated by Western Blot in dichloroacetate untreated and treated HEK 293 cell lysates.

Western Blot Analysis: 0.5 µg/mL of this antibody detected PDHE1-A type I on 10 µg of dichloroacetate untreated and treated HEK 293 cell lysates.

Descrizione del bersaglio

~43 kDa observed

Risultati analitici

Control
Dichloroacetate untreated and treated HEK 293 cell lysates

Altre note

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable