ABN1488

Anti-TIAM-1

from rabbit, purified by affinity chromatography

• Sinonimo/i: T-lymphoma invasion and metastasis-inducing protein 1, TIAM-1
• Codice UNSPSC: 12352203
• eCl@ss: 32160702

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: mouse
• Reattività contro le specie (prevista in base all’omologia): human (based on 100% sequence homology), rat (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology)
• tecniche: western blot: suitable
• N° accesso NCBI: NP_003244
• N° accesso UniProt: Q13009
• Condizioni di spedizione: ambient
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... TIAM1(7074)

Descrizione

Descrizione generale

T-lymphoma invasion and metastasis-inducing protein 1 (UniProt Q13009; also known as TIAM-1) is encoded by the TIAM1 gene (Gene ID 7074) in human. TIAM-1 is a ubiquitously expressed guanine nucleotide exchange factor (GEF) that specifically activates the Rho-like GTPase Rac1 in response to growth factors and cell-substrate interaction. TIAM-1 associates with GTP-bound Ras and functions as an effector that mediates Rac activation by Ras. TIAM-1 promotes the formation of adherens junctions and the induction of an epithelial-like phenotype in mesenchymal cells, and is essential for the formation and maintenance of cadherin-based adhesions. Tiam1-knockdown in mice protects against Ras-induced skin carcinogenesis as a result of increased apoptosis and reduced proliferation/growth of keratinocytes. However, the fewer skin tumors developed progress more frequently to malignancy in Tiam1-knockout mice, indicating that loss of Tiam1 stimulates malignant conversion. Cellular TIAM-1 is targeted for proteasome-dependent degradation by the SCFbTrCP ubiquitin ligase in cooperation with casein kinase 1 and the MEK/ERK pathway upon mitogenic stimulation.

Specificità

This rabbit polyclonal antibody targets a C-terminal region of human and mouse TIAM-1 sequences reported by UniProt (Q13009 and Q60610).

Immunogeno

KLH-conjugated linear peptide corresponding to a C-terminal region sequence of human TIAM-1.

Applicazioni

Anti-TIAM-1 Antibody, Cat. No. ABN1488, is a highly specific rabbit polyclonal antibody, that targets TIAM-1 and has been tested in Western Blotting.

Research Category
Neuroscience

Qualità

Evaluated by Western Blotting in Neuro-2a cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected TIAM-1 in 10 µg of Neuro-2a mouse neuroblastoma cell lysate.

Descrizione del bersaglio

~190 kDa observed. 177.4/170.4 kDa (human isoform 1/2) and 177.4 kDa (mouse) calculated (Met1 removed). Uncharacterized bands may be observed in some lysate(s).

Stato fisico

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% sodium azide

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable