ABE285

Anti-Tristetraprolin Antibody

from rabbit, purified by affinity chromatography

• Sinonimo/i: Tristetraprolin, TTP, G0/G1 switch regulatory protein 24, Growth factor-inducible nuclear protein NUP475, Protein TIS11A, TIS11, Zinc finger protein 36 homolog, Zfp-36
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: human, mouse
• tecniche: RIP: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• N° accesso NCBI: NP_003398
• N° accesso UniProt: P26651
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... ZFP36(7538)

Descrizione

Descrizione generale

Tristetraprolin (TTP, Nup 475, TIS11, G0S24, or ZFP36) is a member of the CCCH tandem zinc finger protein family. It is a nuclear RNA-binding protein that functions as a trans-acting factor. It is involved in post-transcriptional regulation of cytokine genes, most notably Tumor Necrosis Factor alpha (TNF-α). Upon activation by lipopolysaccharides, TTP binds TNF-α mRNA at AU-rich domains in the 3′-untranslated Region (3′-UTR) and induces deadenylation, thereby destabilizing the mRNA and tagging it for degradation. To produce this effect, TTP recruits other trans-acting factors to its C-terminal domain and mRNA-decay enzymes to its N-terminal domain. TTP is deactivated by phosphorylation driven by mitogen-activated protein kinase-activated protein kinase 2 (MK2) and p53. Therefore, TTP/MK2 regulates the biosynthesis of TNF-α and mediates inflammatory responses involving TNF-α. Knockout of TTP in mice produces arthritis, alopecia, dermatitis, among a range of other phenotypes related to overexpression of TNF-α.

Immunogeno

KLH-conjugated linear peptide corresponding to human Tristetraprolin.

Applicazioni

Anti-Tristetraprolin Antibody is an antibody against Tristetraprolin for use in Western Blotting, ICC, Immunoprecipitation, RNA Binding Protein Immunoprecipitation (RIP).

Immunocytochemistry Analysis: A representative lot was used by an independent laboratory to detect Tristetraprolin in human leukocytes. (Brooks, S., et al. (2002). Arthritis and Rheumatism. 46(5):1362–1370.)

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Growth Factors & Receptors

Qualità

Evaluated by Western Blot in A431 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Tristetraprolin on 10 µg of A431 cell lysate.

Descrizione del bersaglio

~40 kDa observed. Uniprot gives a calculated molecular weight of 34 kDa An uncharacterized band appears at ~53 kDa in some lysates.

Stato fisico

Affinity purified

Purified rabbit polyclonal in buffer containing PBS without 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Risultati analitici

Control
A431 cell lysate

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable