ABD43

Anti-Apelin receptor Antibody

from rabbit, purified by affinity chromatography

• Sinonimo/i: Apelin Receptor, Angiotensin Receptor-Like 1, G-Protein Coupled Receptor Apj, G-Protein Coupled Receptor Hg11
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: human, mouse
• Reattività contro le specie (prevista in base all’omologia): rhesus macaque (based on 100% sequence homology), rat (based on 100% sequence homology)
• tecniche: immunohistochemistry: suitable (paraffin); western blot: suitable
• N° accesso NCBI: Np_005152
• N° accesso UniProt: P35414
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... APLNR(187)

Descrizione

Descrizione generale

The Apelin receptor (APJ receptor) is a Gi/o-coupled protein with significant sequence homology to the angiotensin II type 1 (AT1) receptor. It is activated by apelin peptides (12, 17, or 36 amino acids). The effects of the apelin receptor are mediated mainly through the activation of protein kinase C and calcium signaling. The apelin receptor has been detected in the brain, heart, spleen, thymus, ovary, small intestine, and colon. Several studies have suggested that it is involved in regulating cardiovascular function and glucose homeostasis.

Specificità

This antibody recognizes Apelin receptor at the cytoplasmic domain.

Immunogeno

Epitope: Cytoplasmic domain

KLH-conjugated linear peptide corresponding to the cytoplasmic domain of human Apelin receptor.

Applicazioni

Research Category
Stem Cell Research

This Anti-Apelin receptor Antibody is validated for use in Western Blotting, IHC(P) for the detection of Apelin receptor.

Western Blot Analysis: 0.5 µg/mL from a representative lot detected Apelin receptor on 10 µg of human cerebellum tissue lysate.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Apelin receptor in several regions of mouse brain sections.

Qualità

Evaluated by Western Blot in mouse cerebellum tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Apelin receptor on 10 µg of mouse cerebellum tissue lysate.

Descrizione del bersaglio

~62 kDa observed. The calculated molecular weight of this protein is 42 kDa, but can be observed at ~62 kDa due to high glycosylation. (Medhurst, A., et al (2003). Journal of Neurochemistry. 84:1162–1172.)

Stato fisico

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Risultati analitici

Control
Mouse Cerebellum Tissue Lysate

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable