ABC506
Anti-FUNDC1 Antibody
serum, from rabbit
Sinonimo/i:
FUN14 domain-containing protein 1
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
rabbit
Forma dell’anticorpo
serum
Tipo di anticorpo
primary antibodies
Clone
polyclonal
Reattività contro le specie
human, rat, mouse
tecniche
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
dry ice
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... FUNDC1(139341)
Descrizione generale
FUN14 domain-containing protein 1 (FUNDC1) is an important autophagy partner for mitophagy, the process whereby mitochondria get recycled after damage. FUNDC1 is localized to the outer mitochondrial membrane and widely expressed in all cells and appears to act as an activator of hypoxia-induced mitophagy. FUNDC1 interacts directly with important autophagy markers such as MAP1LC3B and GABARAP. Phosphorylation at Tyr-18 inactivates FUNDC1 mitophagy activation ability, and it is this dephosphorylation following hypoxia that initiates the interaction with MAP1LC3 family proteins and mitophagy. Recent research shows that FUNDC1 expression is controlled via the microRNA miRNA-137, and that this microRNA is critical for mitophagy and mitochondrial recycling.
Immunogeno
Epitope: Sigma transmembrane domain
Recombinant protein corresponding to the Sigma transmembrane domain of Human FUNDC1.
Applicazioni
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
This Anti-FUNDC1 Antibody is validated for use in Western Blotting and Immunocytochemistry and Immunoprecipitation for the detection of FUNDC1.
Western Blotting Analysis: A representatitive lot of this antibody was used to detect FUNDC1 in A431, C2C12, HEK293, HeLa, HepG2, L6, Mouse brain, NIH/3T3, PC12, PC3, and RAW264.7 cell lysates. This antibody did not detect FUNDC1 in Human placenta, HUVEC, or Jurkat cell lysates.
Western Blotting Analysis: A representatitive lot of this antibody was used to detect puried FUNDC1 protein from bacteria or HeLa cell lysates (Liu, L., et al. (2012) Nature Cell Biology. 14(2):177-185).
Immunocytochemistry Analysis: A representatitive lot of this antibody was used to detect FUNDC1 in HeLa cells (Liu, L., et al. (2012) Nature Cell Biology. 14(2):177-185.
Immunoprecipitation Analysis: A representatitive lot of this antibody was used to co-immunoprecipitate FUNDC1 with endogenous LC3 in HeLa cells that were exposed to normoxic or hypoxic conditions for 24 hrs (Liu, L., et al. (2012) Nature Cell Biology. 14(2):177-185).
Western Blotting Analysis: A representatitive lot of this antibody was used to detect puried FUNDC1 protein from bacteria or HeLa cell lysates (Liu, L., et al. (2012) Nature Cell Biology. 14(2):177-185).
Immunocytochemistry Analysis: A representatitive lot of this antibody was used to detect FUNDC1 in HeLa cells (Liu, L., et al. (2012) Nature Cell Biology. 14(2):177-185.
Immunoprecipitation Analysis: A representatitive lot of this antibody was used to co-immunoprecipitate FUNDC1 with endogenous LC3 in HeLa cells that were exposed to normoxic or hypoxic conditions for 24 hrs (Liu, L., et al. (2012) Nature Cell Biology. 14(2):177-185).
Qualità
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: A 1:1000 dilution of this antibody detected FUNDC1 in 10 µg of HepG2 cell lysate.
Western Blotting Analysis: A 1:1000 dilution of this antibody detected FUNDC1 in 10 µg of HepG2 cell lysate.
Descrizione del bersaglio
~17 kDa observed.
Uncharacterized bands may be visitible in some lysates.
Uncharacterized bands may be visitible in some lysates.
Stato fisico
Rabbit Polyclonal serum with 0.05% sodium azide.
Unpurified
Stoccaggio e stabilità
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Altre note
Concentration: Please refer to lot specific datasheet.
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Raccomandato
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
A Regulatory Signaling Loop Comprising the PGAM5 Phosphatase and CK2 Controls Receptor-Mediated Mitophagy.
Chen, Guo, et al.
Molecular Cell (2014)
Mitochondrial outer-membrane protein FUNDC1 mediates hypoxia-induced mitophagy in mammalian cells.
Liu, L; Feng, D; Chen, G; Chen, M; Zheng, Q; Song, P; Ma, Q; Zhu, C; Wang, R; Qi et al.
Nature Cell Biology null
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