AB9730
Anti-14-3-3 β Antibody
serum, Chemicon®
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
rabbit
Livello qualitativo
Forma dell’anticorpo
serum
Tipo di anticorpo
primary antibodies
Clone
polyclonal
Reattività contro le specie
rabbit, bovine, human, rat, mouse, sheep
Produttore/marchio commerciale
Chemicon®
tecniche
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
dry ice
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... YWHAB(7529)
Descrizione generale
Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers, thus rendering 14-3-3 as a key multifunctional regulatory molecule. 14-3-3 isomers have been implicated in several neurological disorders: for example isoforms gamma, epsilon and beta are expressed in the cerebrospinal fluid of Creutzfeldt-Jakob patients, but not patients suffering from other neurological conditions such as Alzheimer′s.
The 14-3-3 beta antibody is one of a panel of antibodies that were generated against 14-3-3 isoforms and have demonstrated their potential for use as an ELISA-based diagnostic tool. These antibodies will allow the research to investigate the role of this protein in key cellular processes and neurological disorders such as Creutzfeldt-Jakob, Alzheimer′s and Parkinson′s Disease.
The 14-3-3 beta antibody is one of a panel of antibodies that were generated against 14-3-3 isoforms and have demonstrated their potential for use as an ELISA-based diagnostic tool. These antibodies will allow the research to investigate the role of this protein in key cellular processes and neurological disorders such as Creutzfeldt-Jakob, Alzheimer′s and Parkinson′s Disease.
Specificità
14-3-3 beta. By Western blot the antibody recognizes the ~30 kDa protein.
Immunogeno
Synthetic peptide from the N-terminal area of sheep 14-3-3 beta.
Applicazioni
Detect 14-3-3 β using this Anti-14-3-3 β Antibody validated for use in ELISA, WB, IH.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Neurodegenerative Diseases
Western blot: 1:3,000 using ECL
Immunohistochemistry: 1:300-1:600 on mouse brain tissue which was treated by hydrated autoclaving at 121°C for 15 minutes prior to immunolabeling. Endogenous peroxidase was blocked with hydrogen peroxide in methanol and non-specific labeling with normal goat serum at 1:20 for 5 minutes.
ELISA
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 1:300-1:600 on mouse brain tissue which was treated by hydrated autoclaving at 121°C for 15 minutes prior to immunolabeling. Endogenous peroxidase was blocked with hydrogen peroxide in methanol and non-specific labeling with normal goat serum at 1:20 for 5 minutes.
ELISA
Optimal working dilutions must be determined by end user.
Descrizione del bersaglio
30 kDa
Linkage
Replaces: 04-1089
Stato fisico
Liquid.
Serum
Stoccaggio e stabilità
Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap
Note legali
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Raccomandato
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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I clienti hanno visto anche
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H Martin et al.
FEBS letters, 331(3), 296-303 (1993-10-04)
14-3-3 proteins are apparently ubiquitous eukaryotic proteins that comprise a large number of isoforms. They have been implicated in the regulation of a wide range of biological processes [reviewed in Aitken et al. (1992) Trends Biochem. Sci. 17, 498-501]. We
H C Baxter et al.
Neuroscience, 109(1), 5-14 (2002-01-11)
The appearance of 14-3-3 proteins in the cerebrospinal fluid is characteristic of some neurodegenerative conditions which include sporadic Creutzfeldt-Jakob disease. Although 14-3-3 proteins are physiochemically well characterised and are known to be present in neuronal cells little is known of
Maliha Chaudhri et al.
Biochemical and biophysical research communications, 300(3), 679-685 (2003-01-01)
The 14-3-3 protein family associates with many proteins involved in intracellular signalling. In many cases, there is a distinct preference for a particular isoform(s) of 14-3-3. A specific repertoire of 14-3-3 dimer formation may therefore influence which of the interacting
Petra Steinacker et al.
Molecular and cellular biology, 25(4), 1339-1346 (2005-02-03)
The diagnosis of sporadic Creutzfeldt-Jakob disease (CJD) is based on typical clinical findings and is supported by a positive 14-3-3 Western blot of cerebrospinal fluid. However, it is not clear whether 14-3-3 indicates general neuronal damage or is of pathophysiological
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