AB2219

Anti-Aquaporin 1 Antibody

1.0 mg/mL, Chemicon^®

• Sinonimo/i: Aquaporin-1, AQP-1, Aquaporin-CHIP, Water channel protein for red blood cells and kidney proximal tubule
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: rat, mouse, human
• Reattività contro le specie (prevista in base all’omologia): sheep (based on 100% sequence homology), bovine (based on 100% sequence homology), primate (based on 100% sequence homology)
• Produttore/marchio commerciale: Chemicon^®
• Concentrazione: 1.0 mg/mL
• tecniche: immunohistochemistry: suitable; western blot: suitable
• N° accesso NCBI: NP_036910
• N° accesso UniProt: P29975
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... AQP1(358)

Descrizione

Descrizione generale

Aquaporin 1 is an integral membrane protein which was originally identified in red blood cells and renal proximal tubules. AQP1 is also expressed by the choroid plexus and various other tissues. It forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.

Immunogeno

Epitope: Topological domain

KLH-conjugated linear peptide corresponding to the topological domain of rat Aquaporin 1.

Applicazioni

Anti-Aquaporin 1 Antibody detects level of Aquaporin 1 & has been published & validated for use in ELISA, WB, IH.

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

Western Blotting: 1-10 μg/mL using Chemiluminescence technique. Immunoblots reveal two bands, a lower 28 kDa band corresponding to unglycosylated form and an upper high molecular 35-60 kDa glycosylated component (Denker et al. 1988; Smith & Agre 1991) We recommend the use of 0.5-1.0% milk in all primary/secondary antibody-enzyme conjugate incubations in order to suppress non-specific bands.
Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Aquaporin 1 in human kidney and mouse kidney tissue.

Qualità

Evaluated by Western Blotting in human kidney tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected Aquaporin 1 in 10 µg of human kidney tissue lysate.

Descrizione del bersaglio

28 kDa

Linkage

Replaces: AB3065

Stato fisico

ImmunoAffinity Purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable