AB15348
Anti-FMRFamide Antibody
serum, Chemicon®
Sinonimo/i:
Anti-Anti-FMRFAL
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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Origine biologica
rabbit
Livello qualitativo
Forma dell’anticorpo
serum
Tipo di anticorpo
primary antibodies
Clone
polyclonal
Reattività contro le specie
rat
Produttore/marchio commerciale
Chemicon®
tecniche
immunohistochemistry: suitable
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
dry ice
modifica post-traduzionali bersaglio
unmodified
Categorie correlate
Immunogeno
FMRFamide
Applicazioni
Anti-FMRFamide Antibody is an antibody against FMRFamide for use in IH.
Immunohistochemistry: 1:1,000 - 1:5,000
Application Notes for Immunohistochemistry:
- Suggested perfusion steps:
1) Ca2+-free Tyrode′s solution
2) Paraformaldehyde-picric acid fixative
3) 10% sucrose in PBS as a cryo-protectant
- Rapidly dissect tissue and store overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose.
- Incubate slide-mounted tissue sections with blocking buffer for 1 hour at room temperature.
- Remove blocking buffer and incubate the slides with the primary antibody (AB15348 diluted to desired working concentration in blocking buffer) for 18-24 hours at 2-8°C.
- Remove primary antibody and rinse 3 times in PBS.
- Incubate sections with fluorescently labeled secondary antibody for 60 minutes at room temperature.
Optimal working dilutions must be determined by the end user.
Application Notes for Immunohistochemistry:
- Suggested perfusion steps:
1) Ca2+-free Tyrode′s solution
2) Paraformaldehyde-picric acid fixative
3) 10% sucrose in PBS as a cryo-protectant
- Rapidly dissect tissue and store overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose.
- Incubate slide-mounted tissue sections with blocking buffer for 1 hour at room temperature.
- Remove blocking buffer and incubate the slides with the primary antibody (AB15348 diluted to desired working concentration in blocking buffer) for 18-24 hours at 2-8°C.
- Remove primary antibody and rinse 3 times in PBS.
- Incubate sections with fluorescently labeled secondary antibody for 60 minutes at room temperature.
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Hormones & Receptors
Hormones & Receptors
Stato fisico
Liquid with 0.05% sodium azide.
Serum
Stoccaggio e stabilità
Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Note legali
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Certificati d'analisi (COA)
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