AB10525

Anti-EBF-3 Antibody

from rabbit, purified by affinity chromatography

• Sinonimo/i: Transcription factor COE3, Early B-cell factor 3, EBF-3, Olf-1/EBF-like 2, O/E-2, OE-2
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: mouse
• Reattività contro le specie (prevista in base all’omologia): rat (based on 100% sequence homology), human (based on 100% sequence homology)
• tecniche: dot blot: suitable; immunofluorescence: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• N° accesso NCBI: NP_001106886
• N° accesso UniProt: O08791
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... EBF3(253738) ; mouse ... Ebf3(13593)

Descrizione

Descrizione generale

The family of Early B-cell Factor (EBF) or Collier/Olf-1/EBF (COE) proteins, EBF1, EBF2, and EBF3, are ubiquitous zinc-binding transcription factors found in many species, including human, Drosophila melanogaster, and Caenorhabditis elegans. EBF proteins are capable of forming homo- and heterodimers that bind to DNA at specific sites that include the sequence 5′-ATTCCCNNGGGAATT-3′. EBF1, the prototypical member of this family, may employ epigenetic mechanisms, such as DNA methylation and chromatin remodeling, to regulate genes involved in B-cell development such as Pax5. The second member of this family EBF2 is involved in bone development, adipogenesis, and CNS development, processes which may also hinge on EBF1. EBF3 has been characterized as a tumor suppressor protein, due to its role in regulating multiple genes such as those for cyclins and CDKs which control cell growth, differentiation, and apoptosis. However, emerging evidence indicates that all EBF proteins may play a role in the development of various cancers.

Specificità

This antibody recognizes EB-3 but not EB-1 or EB-2.

Immunogeno

KLH-conjugated linear peptide corresponding to mouse EBF-3.

Applicazioni

Anti-EBF-3 Antibody is an antibody against EBF-3 for use in Western Blotting, IHC(P), Immunofluorescence, Dot Blot.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

Western Blot Analysis: A 1:500 dilution from a representative lot detected EBF-3 in COS-7 cells transfected with EBF-3. (Image courtesy of Dr. Giacomo Consalez, San Raffaele Scientific Institute.)

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected EBF-3 in mouse cryosections of wild type spinal cord tissue. (Image courtesy of Dr. Giacomo Consalez, San Raffaele Scientific Institute.)

Immunofluorescence Analysis: A 1:500 dilution from a representative lot detected EBF-3 in COS-7 cells transfected with EBF expressing plasmids. (Image courtesy of Dr. Giacomo Consalez, San Raffaele Scientific Institute.)

Dot Blot Analysis: EBF-1, EBF-2, and EBF-3 peptides from a representative lot were probed with Anti-EBF-3 (1:100 dilution). No cross reactivity to peptides for EBF-1 & EBF-2 were observed.

Qualità

Evaluated by Western Blot in mouse E16 brain tissue lysate.

Western Blot Analysis: 2 µg/mL of this antibody detected EBF-3 in 10 µg of mouse E16 brain tissue lysate.

Descrizione del bersaglio

~72 kDa observed. An uncharacterized band may appear at ~42, ~55, and ~108 kDa in some lysates.

Stato fisico

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Risultati analitici

Control
Mouse E16 brain tissue lysate

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable