70236-M

BL21(DE3)pLysS Singles Competent Cells - Novagen

Escherichia coli, rod shaped

• Sinonimo/i: BL21(DE3) competent cells, Competent bacterial cells
• Codice UNSPSC: 41106202
• NACRES: NA.54

Proprietà

• product name: BL21(DE3)pLysS Singles Competent Cells - Novagen,
• Origine biologica: Escherichia coli
• Livello qualitativo: 100
• Produttore/marchio commerciale: Novagen^®
• Condizioni di stoccaggio: OK to freeze; avoid repeated freeze/thaw cycles
• Modalità di accrescimento: adherent or suspension
• Morfologia: rod shaped
• tecniche: microbiological culture: suitable
• Trasformazione cellulare: transformation efficiency: >2×10⁷ cfu/μg
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −70°C

Descrizione

Descrizione generale

Genotype: F^-ompT hsdS_B(r_B^- m_B^-) gal dcm (DE3) pLysS (Cam^R)





This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.

BL21 strains are the most widely used hosts for protein expression from pET recombinants and have the advantage of being deficient in both lon and ompT proteases.

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.

Componenti

•11 or 22Singles Competent Cells•2 or 4 × 2 mlSOC Medium•10 µlTest Plasmid

Attenzione

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Note legali

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2