407294

αA-Interferon, Human, Recombinant, E. coli

• Sinonimo/i: IFNA2A, IFNA2B, IFNA2C, rhIFN-αA
• Codice UNSPSC: 12352202
• NACRES: NA.77

Proprietà

• Descrizione: Merck USA index - 14, 4990
• Livello qualitativo: 100
• Saggio: ≥95% (SDS-PAGE)
• Stato: liquid
• Attività specifica: ≥2.8 x 10⁸ units/mg protein; ≥5 x 10⁶ units/mL
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze; avoid repeated freeze/thaw cycles
• tecniche: cell based assay: suitable
• N° accesso NCBI: NM_000605.4
• N° accesso UniProt: P01563
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −70°C
• Informazioni sul gene: human ... IFNA1(3340)

Descrizione

Descrizione generale

Research area: Immunoand cks

Interferons are cytokines secreted by mammalian cells. They are broadly divided into type I– interferon alpha (IFN-α) and interferon beta (IFN-β), type II – interferon-gamma (IFN-γ), and type III – interferon lambda (IFN-λ) based on their structure, function, and receptor specificity. Recombinant αA interferon is a hydrophobic protein belonging to the IFN-α family of proteins.

Applicazioni

Interferon alpha has been used to evaluate its effect on VSV (vesicular stomatitis virus) infection in pancreatic ductal adenocarcinoma (PDA) cell lines and study the relationship between type I IFNsignaling, resistance to VSV infection, and the JAK/STAT (Janus kinase/signal transducers and activators of transcription) signaling pathways in PDA cell lines. It has also been used to study the inhibitory effect of Morbillivirus V Proteins on Interferon Signaling Pathways.

Azioni biochim/fisiol

Recombinant, human αA-interferon expressed in E. coli have antiviral, antiproliferative, antineoplastic, and immunomodulating properties. Recombinant interferon αA (IFN-αA) is used as the first line of treatment for hepatitis C. It also displays a great therapeutic potential with chronic viral hepatitis, polycythemia, leukemia, and various other malignancies. It enhances natural killer cell activity, suppresses bone marrow function, and induces apoptotic cell death in non-melanoma skin cancer.

Attenzione

Toxicity: Standard Handling (A)

Definizione di unità

One unit of interferon per ml of medium will cause a 50% inhibition of VSV-induced cytopathic effect on bovine MDBK cells. Units are determined with respect to the NIH international reference standard for human αA-IFN. Dilute in aqueous buffer containing at least 0.1% BSA or HSA.

Stato fisico

In PBS containing 0.1% BSA.

Nota sulla preparazione

Dilute only in buffers containing protein such as 0.1% BSA.

Ricostituzione

Following initial thaw, aliquot and freeze (-70°C).

Altre note

Fujishima, H., et al. 1998. Int. J. Cancer 76, 423.
Haria, M., and Benfield, P. 1995. Drugs 50, 873.
Familletti, P.C., et al. 1981. Methods Enzymol. 78, 387.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable