34115-M

N-Ethylmaleimide

Sulfhydryl alkylating reagent that inhibits H⁺-ATPase and suppresses the short circuit current (IC₅₀ = 22 µM) in pancreatic duct cells.

• Sinonimo/i: N-Ethylmaleimide, NEM
• Formula empirica (notazione di Hill): C₆H₇NO₂
• Numero CAS: 128-53-0
• Peso molecolare: 125.13
• Numero MDL: MFCD00005509
• Codice UNSPSC: 12352111

Proprietà

• Livello qualitativo: 100
• Saggio: ≥98% (HPLC)
• Forma fisica: solid
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze
• Colore: white
• Solubilità: ethanol: 5 mg/mL
• Temperatura di conservazione: 10-30°C
• InChI: 1S/C6H7NO2/c1-2-7-5(8)3-4-6(7)9/h3-4H,2H2,1H3; HDFGOPSGAURCEO-UHFFFAOYSA-N

Descrizione

Descrizione generale

Sulfhydryl alkylating reagent that inhibits H⁺-ATPase and suppresses the short circuit current (IC₅₀ = 22 µM) in pancreatic duct cells. Inactivates NADP-dependent isocitrate dehydrogenase. Also a potent inhibitor of both Mg²⁺ and Ca²⁺/Mg²⁺-stimulated DNA fragmentation in rat liver nuclei. Stimulates arachidonic acid release through activation of PLA₂ in endothelial cells.

Sulfhydryl alkylating reagent. Has been used for blocking sulfhydryl containing reagents which interfere with glucose oxidase assays. Inactivates NADP-dependent isocitrate dehydrogenase. Endonuclease inhibitor. Also a potent inhibitor of both Mg²⁺ and Ca²⁺/Mg²⁺-stimulated DNA fragmentation in rat liver nuclei.

Azioni biochim/fisiol

Primary Target
H+-ATPase

Target IC₅₀: 22 µM in suppressing short circuit current

Attenzione

Toxicity: Corrosive (L)

Ricostituzione

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Altre note

Huang, Y.C., et al. 1995. Arch. Biochem. Biophys. 316, 485.
Neve, E.P.A., et al. 1995. Biochem. Pharmacol. 49, 57.
Cain, K., et al. 1994. Biochem. Cell Biol.72, 631.
Banger, K.K., et al. 1993. Biochem. J. 290, 199.
Mattingly, J.R., Jr., et al. 1993. J. Biol. Chem. 268, 3925.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Pittogrammi: GHS06,GHS05
• Avvertenze: Danger
• Indicazioni di pericolo: H300,H311,H314,H317
• Consigli di prudenza: P260 - P270 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Classi di pericolo: Acute Tox. 2 Oral - Acute Tox. 3 Dermal - Eye Dam. 1 - Skin Corr. 1B - Skin Sens. 1
• Codice della classe di stoccaggio: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): 163.2 °F - closed cup
• Punto d’infiammabilità (°C): 72.9 °C - closed cup