33-017
PI 3-Kinase HTRF® Assay; 1920 wells
The PI 3-Kinase HTRF Assay is a high-performance assay kit that provides a universal method for testing all Class I PI3-Kinases in a homogeneous format. This pack size has a screening capacity of 5 plates.
Sinonimo/i:
Kinase activity assay
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
About This Item
Codice UNSPSC:
12161503
eCl@ss:
32161000
NACRES:
NA.84
Prodotti consigliati
Livello qualitativo
Reattività contro le specie
mouse, human
Produttore/marchio commerciale
HTRF®
Upstate®
tecniche
activity assay: suitable (kinase)
N° accesso NCBI
N° accesso UniProt
Metodo di rivelazione
fluorometric (HTRF)
Condizioni di spedizione
dry ice
Informazioni sul gene
human ... PIK3CA(5290)
Descrizione generale
The PI3-Kinase HTRF Assay is a high-performance assay kit that provides a universal method for assaying all Class I PI3-Kinases in a homogeneous 384-well format.
Applicazioni
PI 3-Kinase HTRF® Assay; 1920 wells have been used to measure the activity of phosphoinositide-3-kinases (PI3Ks). It has also been used in a biochemical assay to measure the potency and selectivity of LL-00071210 (a selective PI3Kδ inhibitor).
Azioni biochim/fisiol
The PI 3-Kinases catalyze the phosphorylation of the three positions of phosphatidylinositols. The Class I enzymes convert phosphatidylinositol 4,5-bisphosphate (PIP2) to phosphatidylinositol 3,4,5-trisphosphate (PIP3) in the presence of ATP and Mg2+.
Caratteristiche e vantaggi
- Large Stokes shift and time-resolved elements of technology help to reduce compound interference, minimizing concern of false negatives
- Picomole sensitivity of the HTRF assay platform enables the integrity of enzyme kinetics to be retained, ensuring the most physiologically relevant assay parameters.
- Compatible with all Class I phosphoinositide 3-kinase (PI3K) isoforms: enables relative data comparisons and eliminates the need for multiple assay types.
- Reliable, proven assay—based on the technology used in the Millipore, Upstate® KinaseProfiler Service.
- Z` factor routinely exceeds 0.8, ensuring consistent, reliable data.
Confezionamento
Kit capacity: 1920 wells
Componenti
4X Reaction Buffer
PIP2 (Substrate; 1mM stock)
Stop Reagent A
Stop Reagent B
DM A Detection Reagent
DM B Detection Reagent
DM C Detection Reagent
PIP2 (Substrate; 1mM stock)
Stop Reagent A
Stop Reagent B
DM A Detection Reagent
DM B Detection Reagent
DM C Detection Reagent
Note legali
HTRF is a registered trademark of Cis Bio International
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Eye Irrit. 2
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Robert H Sinnamon et al.
Protein expression and purification, 73(2), 167-176 (2010-05-12)
Phosphoinositide 3-kinases have been targeted for therapeutic research because they are key components of a cell signaling cascade controlling proliferation, growth, and survival. Direct activation of the PI3Kalpha pathway contributes to the development and progression of solid tumors in breast
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.