251400

D609, Potassium Salt

Selective inhibitor of phosphatidylcholine (PC)-specific phospholipase C (K_i = 5-10 µM).

• Sinonimo/i: D609, Potassium Salt, Tricyclodecan-9-yl-xanthogenate, K
• Formula empirica (notazione di Hill): C₁₁H₁₅OS₂ · K
• Numero CAS: 83373-60-8
• Peso molecolare: 266.46
• Numero MDL: MFCD00171401
• Codice UNSPSC: 41106500
• NACRES: NA.77

Proprietà

• Livello qualitativo: 100
• Saggio: ≥98% (HPLC)
• Forma fisica: powder
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze; desiccated (hygroscopic)
• Colore: off-white
• Solubilità: DMSO: 25 mg/mL; water: 25 mg/mL
• Condizioni di spedizione: ambient
• Temperatura di conservazione: 2-8°C
• InChI: 1S/C11H16OS2.K/c13-11(14)12-10-5-6-4-9(10)8-3-1-2-7(6)8;/h6-10H,1-5H2,(H,13,14);; YFUFHKMDCZBHMY-UHFFFAOYSA-N

Descrizione

Descrizione generale

Selective inhibitor of phosphatidylcholine (PC)-specific phospholipase C (K_i = 5-10 µM). Does not inhibit phosphatidylinositol-specific phospholipase C, phospholipase A, or phospholipase D. Also reported to inhibit LPS-stimulated ERK activation via its inhibitory effect on PLC. Inhibits angiogenesis by preventing the synthesis of basement membrane.

Selective inhibitor of phosphatidylcholine-specific phospholipase C (PC-PLC) (Bacillus cereus, K_i = 5-10 µM). Does not inhibit (up to 50 µM) phosphatidylinositol-specific phospholipase C, phospholipase A₂, and phospholipase D. Inhibitor of the sphingomyelinase activity. Also inhibits angiogenesis by preventing the synthesis of basement membrane (BM).

Azioni biochim/fisiol

Cell permeable: no

Primary Target
pc-specificphopholipase c

Product does not compete with ATP.

Reversible: no

Target K_i: 5-10 µM against phosphatidylcholine (PC)-specific phospholipase C

Attenzione

Toxicity: Standard Handling (A)

Nota sulla preparazione

tissue culture media should be at neutral pH, since product is active only at or slightly below pH 7.0. The compound is inactive at pH 7.4 and above, and it is hydrolyzed below pH 6.0. HEPES buffer should not be used as it renders D609 toxic.

Ricostituzione

Material is very labile once in solution (1.5 day half-life in tissue culture medium).

Altre note

Monick, M.M., et al. 1999. J. Immunol. 162, 3005.
Lozanski, G., et al. 1997. Biochem. J.328, 271.
Maragoudakis, M.E., et al. 1993. Kidney Int. 43, 147.
Doppler, C., et al. 1992. AIDS Res. Human Retroviruses8, 245.
Schutze, S., et al. 1992. Cell71, 765.
Sauer, G., et al. 1990. Cancer Lett.53, 97.
Muller-Decker, K. 1989. Biochem. Biophys. Res. Commun.162, 198.
Amtmann, E., et al. 1987. Biochem. Pharmacol.36, 1545.
Sauer, G., et al. 1984. Proc. Natl. Acad. Sci. USA81, 3263.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable