219373

Cathepsin G, Human Neutrophil

Cathepsin G, Human Neutrophil, CAS 107200-92-0, is a purified native cathepsin G. Acts as a potent agonist of human platelet activation leading to their aggregation.

• Sinonimo/i: Cathepsin G, Human Neutrophil
• Numero CAS: 107200-92-0
• Classificazione EC (Enzyme Commission): 3.4.21.20 (BRENDA, IUBMB)
• Numero MDL: MFCD00130750
• Codice UNSPSC: 12352202
• NACRES: NA.77

Proprietà

• Origine biologica: human neutrophils
• Livello qualitativo: 100
• Saggio: ≥95% (SDS-PAGE)
• Forma fisica: lyophilized solid (Salt-free)
• Attività specifica: ≥2 units/mg protein
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze
• tecniche: inhibition assay: suitable
• Compatibilità: suitable for molecular biology
• applicazioni: life science and biopharma
• Condizioni di spedizione: ambient
• Temperatura di conservazione: −20°C
• Informazioni sul gene: human ... CTSG(1511)

Descrizione

Descrizione generale

Research area: Cell Signaling

Cathepsin G is stored in its active form in azurophil granules of neutrophils or monocytes. Its mature form contains one potential glycan-binding site and three disulfide bonds.

Applicazioni

Cathepsin G, Human Neutrophil has been used to determine the in vitro bactericidal activities of cathepsin G and for in vitro digestion of plasma/acute wound fluids.

Azioni biochim/fisiol

This protease cleaves the triple-helix structure of type I collagen in a restricted manner and degrades nonhelical regions of type I and II collagens, fibronectin, aggrecan, and elastin. Additionally, cathepsin G activates ProMMP-3 and -8. Cathepsin G serves as a chemoattractant for human phagocytes and enhances the random motility of T cells. Additionally, it initiates chemotaxis, activates ERK1/2 and p38 MAPK, and facilitates the translocation of PKCζ to the cell membrane, without inducing a detectable calcium flux. Other biological functions of cathepsin G include: ECM components and plasma proteins degradation, bactericidal actions, cleavage of inflammatory mediators, conversion of angiotensin I to angiotensin II, platelet activation and induction of airway submucosal gland secretion.

Attenzione

Toxicity: Standard Handling (A)

Definizione di unità

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of Suc-AAPF-pNA per min at 25°C, pH 7.5.

Nota sulla preparazione

Prepared from blood that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitute in 150 mM NaCl, 50 mM sodium acetate buffer, pH 5.5.

Ricostituzione

Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 1 week at 4°C or for up to 6 months at -70°C.

Reconstitute in 50 mM NaOAc and 150 mM NaCl, pH 5.5.

Altre note

Glusa, E., and Adam C. 2001. Por. J. Pharmacol.133, 422.
Shamamian, P., et al. 2001. J. Cell Physiol.189, 197.
Groutas, W.C., et al. 1993. Biochem. Biophys. Res. Commun.197, 730.
Stone, P.J., et al. 1993. Biochem. Biophys. Res. Commun.197, 130.
Groutas, W.C., et al. 1992. Arch. Biochem. Biophys.294, 144.
Maison, C.M., et al. 1991. J. Immunol.147, 921.
Travis, J. 1988. Am. J. Med.84, 37.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable