17-500M
Catch and Release v2.0 Reversible Immunoprecipitation System
Sinonimo/i:
Catch and release system, Immunoprecipitation kit
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About This Item
Codice UNSPSC:
12161503
eCl@ss:
32161000
NACRES:
NB.22
Prodotti consigliati
Reattività contro le specie (prevista in base all’omologia)
all
Livello qualitativo
Produttore/marchio commerciale
Catch And Release
Upstate®
tecniche
activity assay: suitable (IP-kinase)
immunoprecipitation (IP): suitable
western blot: suitable
Condizioni di spedizione
wet ice
Descrizione generale
Features & Benefits:
Reproducible: eliminates aspiration steps that typically lead to diminished/lost sample
Convenient: spin-column format dramatically simplifies sample handling
Reversible: simple elution of antigen:antibody complex in either native or denatured form
Use:
This kit allows for quick and reproducible immunoprecipitation (IP) by using a spin column. The system is more reproducible than regular IP′s, which are problematic with regards to washing the protein A/G agarose without disrupting the agarose bed. The binding of the antibody/antigen complex in Catch and Release is reversible, and elution of the immune complex can occur with native or denaturing buffers. The system has been tested successfully with rabbit, mouse, sheep and goat antibodies. IP using human IgG1-4 should be suitable. IP using chicken antibodies or human IgA, IgD, IgE or IgM is not recommended with this kit. Please read the enclosed product manual before use.
Reproducible: eliminates aspiration steps that typically lead to diminished/lost sample
Convenient: spin-column format dramatically simplifies sample handling
Reversible: simple elution of antigen:antibody complex in either native or denatured form
Use:
This kit allows for quick and reproducible immunoprecipitation (IP) by using a spin column. The system is more reproducible than regular IP′s, which are problematic with regards to washing the protein A/G agarose without disrupting the agarose bed. The binding of the antibody/antigen complex in Catch and Release is reversible, and elution of the immune complex can occur with native or denaturing buffers. The system has been tested successfully with rabbit, mouse, sheep and goat antibodies. IP using human IgG1-4 should be suitable. IP using chicken antibodies or human IgA, IgD, IgE or IgM is not recommended with this kit. Please read the enclosed product manual before use.
Applicazioni
Catch and Release v2.0 should not be used with proteins containing a 6-His tag.
Confezionamento
50 immunoprecipitations
Componenti
Catch and Release Wash Buffer, 10X (Cat.# 20-210)
Catch and Release Non-denaturing Elution Buffer, 4X (Cat.# 20-209)
Catch and Release Denaturing Elution Buffer, 1X (Cat.# 20-284)
20-285
20-216
Catch and Release Capture Tubes
Catch and Release Non-denaturing Elution Buffer, 4X (Cat.# 20-209)
Catch and Release Denaturing Elution Buffer, 1X (Cat.# 20-284)
20-285
20-216
Catch and Release Capture Tubes
Qualità
routinely evaluated by immunoprecipitation.
Stoccaggio e stabilità
All components to be stored at 4°C except the Catch and Release Capture Tubes which are stored at room temperature. Components are stable for 6 months from date of shipment.
Note legali
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Avvertenze
Danger
Indicazioni di pericolo
Classi di pericolo
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Repr. 1B - Skin Corr. 1C
Codice della classe di stoccaggio
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
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