171272

AMPK Activator VI, RSVA314

The AMPK Activator VI, RSVA314 modulates the biological activity of AMPK. This small molecule/inhibitor is primarily used for Biochemicals applications.

• Sinonimo/i: AMPK Activator VI, RSVA314, RSVA314, AMPK Signaling Activator VI
• Formula empirica (notazione di Hill): C₁₈H₂₁N₃O₃
• Numero CAS: 316130-82-2
• Peso molecolare: 327.38
• Codice UNSPSC: 12352200
• NACRES: NA.51

Proprietà

• Livello qualitativo: 100
• Saggio: >90% (HPLC)
• Forma fisica: solid
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze; protect from light
• Colore: yellow
• Solubilità: DMSO: 50 mg/mL
• Condizioni di spedizione: ambient
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

A cell permeable hydrozide that activates AMPK indirectly via a CaMKKβ-dependent pathway similar to resveratrol (Cat. No. 554325), which results in the phosphorylation of Thr-172 on the AMPKα2 subunit. This activator promotes lysosomal amyloid-β degradation in 3 cell lines nearly 40 times more potently than resveratrol with an IC₅₀ of 1-2 µM. Treatment with RSVA314 at 1-3 µM results in a dose-dependent inhibition of the phosphorylation of 3 proteins downstream from mTOR activation including p70S6K, eukaryotic elongation factor 2 kinase (eEF2K), and S6 ribosomal protein, and promotes a robust increase in the phosphorylation of the protein raptor at Ser-792 in western blots. At the same concentrations, it elicits a strong, dose-dependent conversion of the light chain 3 (LC3) protein, a marker involved with autophagy induction, from LC3-I and LC3-II, also in western blots.

A cell permeable hydrozide that activates AMPK indirectly via a CaMKKβ-dependent pathway similar to resveratrol (Cat. No. 554325), which results in the phosphorylation of Thr-172 on the AMPKα2 subunit. This activator promotes lysosomal amyloid-β degradation in 3 cell lines nearly 40 times more potently than resveratrol with an IC₅₀ of 1-2 µM. Treatment with RSVA314 at 1-3 µM results in a dose-dependent inhibition of the phosphorylation of 3 proteins downstream from mTOR activation including p70S6K, eukaryotic elongation factor 2 kinase (eEF2K), and S6 ribosomal protein, and promotes a robust increase in the phosphorylation of the protein raptor at Ser-792 in western blots. At the same concentrations, it elicits a strong, dose-dependent conversion of the light chain 3 (LC3) protein, a marker involved with autophagy induction, from LC3-I and LC3-II, also in western blots.

Confezionamento

Packaged under inert gas

Attenzione

Toxicity: Standard Handling (A)

Altre note

Vingtdeux, V., et al. 2011. FASEB J.25, 219.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable