164545

7-Amino-4-methylcoumarin

≥98% (HPLC), solid

• Sinonimo/i: 7-Amino-4-methylcoumarin, AMC, Coumarin 120
• Formula empirica (notazione di Hill): C₁₀H₉NO₂
• Numero CAS: 26093-31-2
• Peso molecolare: 175.18
• Numero MDL: MFCD00006868
• Codice UNSPSC: 12352200
• NACRES: NA.21

Proprietà

• product name: 7-Amino-4-methylcoumarin, Reagent used to prepare fluorogenic 7-amido-4-methylcoumarin (AMC) based substrates for the detection of proteolytic enzyme activity.
• Livello qualitativo: 100
• Saggio: ≥98% (HPLC)
• Forma fisica: solid
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze; protect from light
• Colore: yellow
• Solubilità: DMSO: 10 mg/mL; acetone: 10 mg/mL; DMF: soluble
• Fluorescenza: λ_ex 365-380 nm; λ_em 430-460 nm
• Condizioni di spedizione: ambient
• Temperatura di conservazione: 2-8°C
• InChI: 1S/C10H9NO2/c1-6-4-10(12)13-9-5-7(11)2-3-8(6)9/h2-5H,11H2,1H3; GLNDAGDHSLMOKX-UHFFFAOYSA-N

Descrizione

Descrizione generale

Reagent used to prepare fluorogenic 7-amido-4-methylcoumarin (AMC) based substrates for the detection of proteolytic enzyme activity. Useful as a reference standard in enzyme assays.

Reagent used to prepare fluorogenic 7-amino-4-methylcoumarin (AMC) based substrates for the detection of proteolytic enzymes (excitation maximum: ~365-380 nm; emission maximum: ~430-460 nm). Also useful as a reference standard in enzyme assays.

Azioni biochim/fisiol

Cell permeable: no

Primary Target
Detection of proteolytic enzyme activity

Product does not compete with ATP.

Reversible: no

Confezionamento

Packaged under inert gas

Attenzione

Toxicity: Irritant (B)

Ricostituzione

Following reconstitution, aliquot and freeze (-20°C). DMF and DMSO stock solutions are stable for up to 6 months at -20°C. Acetone stock solutions are stable for up to 1 week at 4°C or -20°C.

Altre note

Kanaoka, Y., et al. 1982. Chem. Pharm. Bull. 30, 1485.
Zimmerman, M., et al. 1977. Anal. Biochem. 78, 47.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable