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Supelco

Chromolith® WP 300 Epoxy (monolithic) HPLC Columns

L × I.D. 25 mm x 4.6 mm HPLC Column

Sinonimo/i:

Chromolith® WP 300 Epoxy 25-4.6 HPLC column

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About This Item

Codice UNSPSC:
41115700
NACRES:
SB.52

product name

Chromolith® WP 300 Epoxy, L × I.D. 25 mm x 4.6 mm HPLC column

Materiali

PEEK column

Livello qualitativo

Nome Commerciale

Chromolith®
Chromolith®

Caratteristiche

endcapped: no
endcapped: no

Confezionamento

1 ea of

Grado di funzionalizzazione

5-6% Carbon loading

Parametri

2 - 60 °C temp. range
2-60 °C temp. range
200 bar pressure
200 bar pressure

tecniche

HPLC: suitable
HPLC: suitable
LC/MS: suitable

Lungh. × diam. int. colonna

2.5 cm × 4.6 mm

Area superficiale

120 m2/g

Matrice

monolithic silica
monolithic silica

Gruppo funzionale matrice

Epoxy bonding phase
Epoxy bonding phase

Dimensione pori

2 μm macroporosity
2 μm macroporosity
30 nm mesoporosity
30 nm mesoporosity

operating pH range

1.5-7.5
1.5-7.5

Temperatura di conservazione

no temp limit

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Descrizione generale

Chromolith® WP 300 Epoxy columns are specially designed for the user-specific immobilization of ligands and their later application in HPLC. The unique bimodal pore structure of silica monoliths allows efficient coupling independent of molecule size. The wider mesopores also enable the use of proteins and antibodies as both ligand immobilized on the column, and later analyte separated by an immobilized column.
Potential applications: attach trypsin to obtain HPLC column-protein digestion reactor; attach protein and measure other protein interaction with the attached one; attach any chiral selector to obtain a chiral column; attach any affinity ligand to obtain custom made affinity column, etc.

Chromolith® WP 300 Epoxy 25-4.6 columns are made of monolithic silica with a characteristic bimodal pore structure.

Key Benefits:
—Easy to perform immobilization of ligand opens up limitless customization options
—Monolithic format allows for high flow rates at low backpressure, enabling high throughput

Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns

Chromolith® WP 300 Epoxy is available in 2 mm I.D. providing improved efficiency, sensitivity, and enhanced MS-compatibility.
Flyer: Create Your Own Column Chemistry - Chromolith® WP 300 Epoxy 2 mm I.D. HPLC Columns

All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

Note legali

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

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Articoli

Silica monoliths with larger mesopores are a powerful tool for the fast separation of biomolecules including mAbs. The immobilization of protein A on epoxy-modified widepore (WP) silica rods enabled highly reproducible and fast, one minute, separations of mAbs. The Chromolith® WP 300 is also available with a protein A phase.

Contenuto correlato

Analytical Chromolith® HPLC columns for Bio applications, 300A mesopores: RP-18, RP-8, RP-4, Prot-A, Epoxy

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