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1.52020

Supelco

Chromolith® HighResolution RP-18 endcapped (monolithic) HPLC Columns

L × I.D. 25 mm x 4.6 mm HPLC Column

Sinonimo/i:

Chromolith® HighResolution RP-18 endcapped 25-4.6 HPLC column

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About This Item

Codice UNSPSC:
41115709
NACRES:
SB.52

product name

Chromolith® HighResolution RP-18 endcapped, L × I.D. 25 mm x 4.6 mm HPLC column

Materiali

PEEK column

Livello qualitativo

agenzia

suitable for ISO/CEN 6304.09
suitable for USP L1

Nome Commerciale

Chromolith®

Caratteristiche

endcapped
endcapped

Confezionamento

1 ea of

Grado di funzionalizzazione

15% Carbon loading
18% Carbon loading

Parametri

200 bar pressure
200 bar pressure
45 °C max. temp.
50 °C max. temp.

tecniche

HPLC: suitable
HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)
mass spectrometry (MS): suitable (High)

Lungh. × diam. int. colonna

2.5 cm × 4.6 mm

Area superficiale

250 m2/g × 250 m2/g

Matrice

highly porous
monolithic silica
monolithic silica

Gruppo funzionale matrice

C18 bonding phase, octadecylsilane (ODS)
C18 bonding phase

Dimensione pori

1.15 μm macroporosity
1.5 μm macroporosity
15 nm mesoporosity
15 nm mesoporosity

operating pH range

2-7.5
2-7.5

Tecnica di separazione

reversed phase

Temperatura di conservazione

no temp limit

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Descrizione generale

Chromolith® columns are made of monolithic silica with a characteristic bimodal pore structure.

Chromolith® HighResolution RP-18 endcapped columns have macropores with 1.15 μm diameter, giving a column efficiency exceeding 140,000 plates/meter. The mesopores are 15 nm (150 Å), the surface modification is octadecylsilane with full end-capping.

Chromolith® HighResolution RP-18 endcapped columns fall under the USP L1 Classification. The column efficiency is comparableto a 2.7 to 3 μm particle size fully porous particulate column. Chromolith® HighResolution RP-18 endcapped columns are perfectly suitable for LC-MS applications.
The column dimension of 25 mm length and 4.6 mm I.D. is the best choice for very rapid separations on any HPLC System and rapid method development.

The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity

The recommended Guard-columns are:
Chromolith® HighResolution RP-18 endcapped 5-4.6 guard cartridges (3 pieces) [1.52025] with the Chromolith® Guard Cartridge Holder 5 mm x 4.6 mm [1.52032]
The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467]

Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns

All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

Chromolith® HighResolution RP-18 endcapped columns are also available in 2 mm I.D. column dimension for perfect fit to UHPLC and UHPLC-MS needs.
Flyer: UHPLC-like performance - Rapid and Robust at Low Pressure - Chromolith® HighResolution RP-18 endcapped 2 mm I.D. HPLC Columns

Risultati analitici

Pressure: 10 - 30 bar
Performance (anthracene)
Theoretical Plates (N/m) (Anthracene): ≥ 110000
Capacity factor (Anthracene): 3.3 - 4.6
Tailing (USP): 0.90 - 1.40 %
Selectivity
separation factor (anthracene/progesterone): 1.85 - 2.35

Note legali

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificati d'analisi (COA)

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Articoli

Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.

Chromolith® HPLC and UHPLC columns are made from monolithic silica with a bimodal pore structure using sol-gel technology.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

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