1.50481
SeQuant™ ZIC®-HILIC 200Å (5 µm) HPLC Columns
L × I.D. 150 mm x 0.3 mm capillay Column
About This Item
Prodotti consigliati
product name
SeQuant™ ZIC-HILIC™ (5µm,200Å) 150 x 0.3 mm, suitable for HPLC
Materiali
stainless steel column
Livello qualitativo
agenzia
suitable for USP L114
Nome Commerciale
SeQuant™
Caratteristiche
endcapped: no
Parametri
400 bar pressure
70 °C max. temp.
tecniche
HPLC: suitable
LC/MS: suitable (Capillary LC)
mass spectrometry (MS): suitable (High)
Lungh. × diam. int. colonna
15 cm × 0.3 mm
Matrice
fully porous particle
Gruppo funzionale matrice
ZIC-HILIC bonding phase
Dimensione particelle
5 μm
Dimensione pori
200 Å pore size
pH di lavoro
2-7.5
Tecnica di separazione
hydrophilic interaction (HILIC)
Temperatura di conservazione
no temp limit
Descrizione generale
The silica-based ZIC®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.
The Pore size of 200 Å makes this column perfectly suitable for the separation of larger polar molecules under HILIC conditions.
The column dimension of 150 mm length x 0.3 mm ID is the optimal choice for the Capillary Chromatography separation of complex samples with high sensitivity.
Thanks to their favorable retention and robust performance, SeQuant™ HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant™ ZIC®-HILIC for analysis of melamine and related substances. SeQuant™ HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.
Learn more about the Supelco HPLC Column portfolio and Method development:
Brochure: HPLC and UHPLC Column Selection Guide
Brochure: A Practical Guide to HPLC - Method Development Guide
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