13-173

MBP, recombinant

• Sinonimo/i: myelin basic protein
• Codice UNSPSC: 12352200
• eCl@ss: 32160405
• NACRES: NA.41

Proprietà

• Origine biologica: bovine
• Livello qualitativo: 100
• Forma fisica: liquid
• PM: M_w ~47 kDa
• Produttore/marchio commerciale: Upstate^®
• Concentrazione: 1 mg/mL
• tecniche: activity assay: suitable
• N° accesso NCBI: NM_001025081.1; NM_001025090.1; NM_001025092.1; NM_001025094.1; NM_001025098.1; NM_001025100.1; NM_001025101.1; NM_002385.2
• N° accesso UniProt: P02686
• Condizioni di spedizione: dry ice

Descrizione

Descrizione generale

Product Source: Recombinant fusion protein containing bovine myelin basic protein (MBP) with an N-terminal GST tag and a C-terminal 6His tag, expressed in E. coli.

Qualità

Routinely evaluated by kinase assay

Stato fisico

500 µg recombinant MBP in 500 µl of 50mM Tris-HCl, pH 7.5, 150mM NaCl, 500 mM imidazole, 1 mM PMSF, and 10% glycerol. Frozen solution.

ProBond Resin chromatography

Stoccaggio e stabilità

Stable for 2 years at -20C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Risultati analitici

Specific Activity: This lot was successfully phosphorylated in a non-radioactive kinase assay using recombinant active MAP Kinase 2/Erk2 (Catalog # 14-173). MBP phosphorylation was detected by immunoblot analysis using anti-phospho-MBP (Catalog # 05-429). Immunoblot shown to the right.

Altre note

Purity information is lot specific, please consult the certificate of analysis

Note legali

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable