1.15943

Methylene blue (C.I.52015)

for microscopy Certistain^®

• Sinonimo/i: Methylene Blue trihydrate, Methylthioninium chloride, 3,7-Bis(dimethylamino)phenothiazinium chloride, Tetramethylthionine chloride
• Formula empirica (notazione di Hill): C₁₆H₁₈ClN₃S · 3H₂O
• Numero CAS: 7220-79-3
• Peso molecolare: 373.90
• Beilstein: 3599847
• Numero MDL: MFCD00012111
• Codice UNSPSC: 41116130
• Numero indice EU: 200-515-2

Proprietà

• Livello qualitativo: 400
• Forma fisica: solid
• Potenza: 1180 mg/kg LD₅₀, oral (Rat)
• Composizione: Dye, ≥82% spectrophotometric assay
• pH: 3 (20 °C, 10 g/L in H₂O)
• Punto di fusione: 190 °C (dec.) (lit.)
• Solubilità: 50 g/L
• Densità: 1.757 g/cm³ at 22 °C
• Densità bulk: 400‑600 kg/m³
• εmax: 2250-2750 at 660-665 nm in 50% ethanol at 0.003 g/L
• applicazioni: diagnostic assay manufacturing; hematology; histology
• Temperatura di conservazione: 2-30°C
• Stringa SMILE: [Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C
• InChI: 1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1; CXKWCBBOMKCUKX-UHFFFAOYSA-M

Descrizione

Descrizione generale

Methylene blue (C.I. 52015) for microscopy Certistain^® is a dry dye that is used in bacteriology for an overview stain and as counterstaining for Ziehl-Neelsen method in sample material of human origin. Bacteria appear blue. The dye has a further important application in hematology: Methylene blue and eosin are the main components of many staining solutions and dye mixtures. Certistain^® is the product group for seleceted high quality dyes that fulfill all quality requirements of IVD and CE registration. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Risultati analitici

Dye content (spectrophotometrically): ≥ 82 %
Identity
- Chloride:
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (ethanol 50 %): 660 - 665 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.003 g/l; ethanol 50 %): 2250 - 2750
TLC-Test: passes test
Loss on drying (110 °C): 10 - 15 %
Suitability for microscopy: passes test

Note legali

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Pittogrammi: GHS07
• Avvertenze: Warning
• Indicazioni di pericolo: H302
• Consigli di prudenza: P264 - P270 - P301 + P312 - P501
• Classi di pericolo: Acute Tox. 4 Oral
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable