110145

D-erythro-Sphingosine, N-Acetyl-

Biologically active, cell-permeable, non-physiological ceramide analog.

• Sinonimo/i: D-erythro-Sphingosine, N-Acetyl-, C₂ Ceramide
• Formula empirica (notazione di Hill): C₂₀H₃₉NO₃
• Numero CAS: 3102-57-6
• Peso molecolare: 341.53
• Codice UNSPSC: 12352211
• NACRES: NA.77

Proprietà

• Livello qualitativo: 100
• Saggio: ≥98% (TLC)
• Forma fisica: solid
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze
• Colore: white to off-white
• Solubilità: DMSO: 25 mg/mL; ethanol: 5 mg/mL; methanol: 5 mg/mL
• Condizioni di spedizione: ambient
• Temperatura di conservazione: −20°C
• InChI: 1S/C20H39NO3/c1-3-4-5-6-7-8-9-10-11-12-13-14-15-16-20(24)19(17-22)21-18(2)23/h15-16,19-20,22,24H,3-14,17H2,1-2H3,(H,21,23)/b16-15+/t19-,20+/m0/s1; BLTCBVOJNNKFKC-QUDYQQOWSA-N

Descrizione

Descrizione generale

Biologically active, cell-permeable, non-physiological ceramide analog. Inhibits cell growth and induces apoptosis in HL-60 cells. Induces intranucleosomal DNA fragmentation. An activator of heterotrimeric protein phosphatase 2A (PP2A). Stimulates a cytosolic serine/threonine protein phosphatase in T9 cells at concentrations as low as 100 nM. Activates stress activated protein kinase (SAPK) in HL-60 human promyelocytic cells.

Biologically active, cell-permeable, non-physiological ceramide analog. Inhibits cell growth and induces apoptosis of HL-60 cells. Induces intranucleosomal DNA fragmentation. An activator of heterotrimeric protein phosphatase 2A. Stimulates a cytosolic serine/threonine protein phosphatase in T9 cells at concentrations as low as 100 nM. Activates stress-activated protein (SAP) kinase in HL-60 human promyelocytic cells.

Azioni biochim/fisiol

Cell permeable: yes

Primary Target
Inhibits cell growth and induces apoptosis in HL-60 cells

Product does not compete with ATP.

Reversible: no

Attenzione

Toxicity: Irritant (B)

Nota sulla preparazione

When diluting into aqueous solution, sonication may be necessary to achieve a uniform suspension.

Ricostituzione

Following reconstitution, aliquot and freeze (-20°C). DMSO stock solutions are stable for up to 1 month at -20°C. Alcohol stock solutions are stable for <1 week at -20°C.

Altre note

Westwick, J.K., et al. 1995. J. Biol. Chem.270, 22689.
Cifone, M.G., et al. 1994. J. Exp. Med.180, 1547.
Hannun, Y.A. 1994. J. Biol. Chem. 269, 3125.
Bielawska, A., et al. 1993. J. Biol. Chem.268, 26226.
Obeid, L.M., et al. 1993. Science259, 1769.
Ballou, L.R., et al. 1992. J. Biol. Chem.267, 20044.
Dobrowsky, R.T., and Hannun, Y.A. 1992. J. Biol. Chem.267, 5048.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable