07-260
Anti-AUF1 Antibody
Upstate®, from rabbit
Sinonimo/i:
Anti-AUF1, Anti-AUF1A, Anti-HNRPD, Anti-P37, Anti-hnRNPD0
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
rabbit
Livello qualitativo
Forma dell’anticorpo
purified immunoglobulin
Tipo di anticorpo
primary antibodies
Clone
polyclonal
Reattività contro le specie
human, mouse, rat
Produttore/marchio commerciale
Upstate®
tecniche
western blot: suitable
Isotipo
IgG
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
dry ice
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... HNRNPD(3184)
mouse ... Hnrnpd(11991)
rat ... Hnrnpd(79256)
Descrizione generale
A+U Rich RNA Binding Factor (AUF1) is comprised of four isoforms of 37, 40, 42, and 45 kDa. Although the role of each isoform has yet to be fully characterized, a direct correlation has been observed between each AUF1 isoform’s binding affinity and its RNA destabilizing activity toward different AREs (A + U rich element in the 3’ untranslated region), with the isoforms p37 and p42 being the most effective.
AUF1 is a bcl2 mRNA binding protein and potentially all its isoforms are able to form complexes with the bcl2 ARE. ARE mediated bcl2 mRNA downregulation during apoptosis involves AUF1 and suggest different roles for its four isoforms.
AUF1 is a bcl2 mRNA binding protein and potentially all its isoforms are able to form complexes with the bcl2 ARE. ARE mediated bcl2 mRNA downregulation during apoptosis involves AUF1 and suggest different roles for its four isoforms.
Specificità
Recognizes AUF1 isoforms.
Immunogeno
Purified AUF1
Applicazioni
Anti-AUF1 Antibody is a high quality Rabbit Polyclonal Antibody for the detection of AUF1 & has been validated in WB.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
RNA Binding Protein (RBP)
Transcription Factors
RNA Binding Protein (RBP)
Qualità
routinely evaluated by immunoblot on RIPA lysates from HeLa nuclear extract or A431 cells
Descrizione del bersaglio
37, 40, 42, 45 kDa
Stato fisico
Format: Purified
Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
Protein A purified
Stoccaggio e stabilità
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Risultati analitici
Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.
Altre note
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Note legali
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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Destabilization of interleukin-6 mRNA requires a putative RNA stem-loop structure, an AU-rich element, and the RNA-binding protein AUF1.
Paschoud, S; Dogar, AM; Kuntz, C; Grisoni-Neupert, B; Richman, L; Kuhn, LC
Molecular and cellular biology null
Cigarette smoke enhances human rhinovirus-induced CXCL8 production via HuR-mediated mRNA stabilization in human airway epithelial cells.
Hudy, MH; Proud, D
Respiratory Research null
Steven J Cok et al.
The Journal of biological chemistry, 279(9), 8196-8205 (2003-12-10)
RAW 264.7 cells rapidly induce cyclooxygenase-2 (COX-2) in response to lipopolysaccharide treatment. Part of the increased COX-2 expression occurred through post-transcriptional mechanisms mediated through specific regions of the 3'-untranslated region (UTR) of the message. The proximal region of the 3'-UTR
The role of HuR in the post-transcriptional regulation of interleukin-3 in T cells.
Gonzalez-Feliciano, JA; Hernandez-Perez, M; Estrella, LA; Colon-Lopez, DD; Lopez et al.
Testing null
mRNA degradation plays a significant role in the program of gene expression regulated by phosphatidylinositol 3-kinase signaling.
Graham, JR; Hendershott, MC; Terragni, J; Cooper, GM
Molecular and cellular biology null
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