07-2113

Anti-phospho-Ribosomal Protein S6 Antibody (Ser240/Ser244)

from rabbit, purified by affinity chromatography

• Sinonimo/i: 40S ribosomal protein S6, Phosphoprotein NP33
• Codice UNSPSC: 12352203
• eCl@ss: 32160702

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: human
• Reattività contro le specie (prevista in base all’omologia): bovine (based on 100% sequence homology), rat (based on 100% sequence homology), Xenopus (based on 100% sequence homology), mouse (based on 100% sequence homology), zebrafish (based on 100% sequence homology)
• tecniche: immunocytochemistry: suitable; western blot: suitable
• N° accesso NCBI: NP_001001
• N° accesso UniProt: P62753
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: phosphorylation (pSer240/pSer244)
• Informazioni sul gene: human ... RPS6(6194)

Descrizione

Descrizione generale

S6 (Ribosomal protein S6, RpS6, 40S ribosomal protein S6) is known to play a role in cell growth and proliferation. It lays downstream of both the PI3K/Akt/mTOR pathway as a substrate of p70 S6 kinase and the Ras/Erk pathway as a substrate of RSK. S6 is phosphorylated in response to stimulation by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. There are two major areas of S6 phosphorylation; Ser235/Ser236 that are known to be phosphorylated by both p70S6 Kinase as well as RSK and Ser240/244 which is thought to only be phosphorylated by p70S6K.

Specificità

This antibody recognizes Ribosomal Protein S6 phosphorylated at Ser240/Ser244.

Immunogeno

Epitope: Phosphorylated Ser240/Ser244

KLH-conjugated linear peptide corresponding to human Ribosomal Protein S6 phosphorylated at Ser240/Ser244.

Applicazioni

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Ribosomal Protein S6 in A431 cells and PMA treated A431 cells.

Research Category
Signaling

Research Sub Category
PI3K, Akt, & mTOR Signaling

This phospho-Ribosomal Protein S6 antibody is validated for use in WB & ICC for the detection of the phospho-Ribosomal Protein S6 protein.

Qualità

Evaluated by Western Blot in PMA treated and untreated A431 cell lysates.

Western Blot Analysis: 2 µg/mL of this antibody detected Ribosomal Protein S6 on 10 µg of PMA treated and untreated A431 cell lysates.

Descrizione del bersaglio

~32 kDa observed

Stato fisico

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Risultati analitici

Control
PMA treated and untreated A431 cell lysates

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable