07-1708

Anti-FOXH1 Antibody

from rabbit, purified by affinity chromatography

• Sinonimo/i: Forkhead activin signal transducer 1, Forkhead activin signal transducer 2, TGF-beta/activin signal transducer, forkhead activin signal transducer-1, forkhead box H1
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: human
• Reattività contro le specie (prevista in base all’omologia): mouse
• tecniche: immunocytochemistry: suitable
• N° accesso NCBI: NM_007989
• N° accesso UniProt: O75593
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... FOXH1(8928) ; mouse ... Foxh1(14106)

Descrizione

Descrizione generale

Forkhead box H1 (FOXH1) is a member of the HNF–3FKH family of transcriptional activators. FOXH1 is a winged helix with two loops on the C-terminal side expressed in all human tissue. FOXH1 is an embryonic transcription regulator and is an essential transcriptional co-activator that binds with SMAD2 to activate activin, and therefore induce the gooseciod (GSC) promoter. After FOXH1 binds to SMAD4, activin response element is activated and TGF-β is stimulated. FOXH1 also interacts with NKX2-5 to form an essential component of anterior heart field development. FOXH1 is associated with complex brain malfunctions due to incomplete cleavage of the prosencephalon, and in developmental delays resulting in facial abnormalities such as cyclopia and severe cleft lip or palate.

Specificità

This antibody recognizes FOXH1.

Immunogeno

Synthetic to peptide corresponding to FOXH1.

Applicazioni

Anti-FOXH1 Antibody is a Rabbit Polyclonal Antibody for detection of FOXH1 also known as Forkhead activin signal transducer, TGF-beta/activin signal transduce & has been validated in ICC.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Qualità

Evaluated by immunocytochemistry on NIH/3T3, HeLa, and A431 cells.

Immunocytochemistry:
1:500 dilution of this antibody was used to detect FOXH1 in NIH/3T3, HeLa and A431 cells.

Descrizione del bersaglio

38 kDa

Stato fisico

Affinity purified

Purified rabbit polyclonal antibody precipitated in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.

Stoccaggio e stabilità

Stable for 1 year at 2-8ºC from date of receipt.
To reconstitute the antibody, centrifuge the antibody at a moderate speed (5,000 rpm) for 5 minutes. Carefully remove the ammonium sulfate/PBS buffer solution and discard; 10μL of residual ammonium sulfate solution will not affect the re-suspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur. Re-suspend the antibody pellet in 100L either standard PBS or TBS (pH 7.3-7.5). DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to re-suspend are normal. Vials are overfilled to compensate for any losses.

Risultati analitici

Control
NIH/3T3, HeLa, and A431 cells.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable