07-1286
Anti-ATM Antibody
Upstate®, from rabbit
Sinonimo/i:
A-T, mutated, AT mutated, ataxia telangiectasia mutated, ataxia telangiectasia mutated (includes complementation groups A, C and D), ataxia telangiectasia mutated protein, ATM, human phosphatidylinositol 3-kinase homolog, serine-protein kinase ATM, TEL1,
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
rabbit
Livello qualitativo
Forma dell’anticorpo
affinity isolated antibody
Tipo di anticorpo
primary antibodies
Clone
polyclonal
Purificato mediante
affinity chromatography
Reattività contro le specie
mouse, human
Reattività contro le specie (prevista in base all’omologia)
horse (based on 100% sequence homology), bovine (based on 100% sequence homology), rat (based on 100% sequence homology)
Produttore/marchio commerciale
Upstate®
tecniche
immunocytochemistry: suitable
western blot: suitable
Isotipo
IgG
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
wet ice
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
bovine ... Atm(526824)
human ... ATM(472)
mouse ... Atm(11920)
rat ... Atm(300711)
Descrizione generale
ATM (Ataxia Telangiectasia Mutated kinase) and ATR (Ataxia Telangiectasia and Rad3-related kinase) are related kinases that regulate cell cycle checkpoints and DNA repair. ATM is activated in response to DNA damage and serves to arrest further cell division before the damage can be repaired. Mutation in the ATM gene results in the autosomal recessive disease ataxia telangiectasia (AT). The identified substrates for ATM include p53, p95/NBS1, MDM2, Chk2, BRCA1, CtIP, 4E-BP1 and Chk1. ATM activates p53, increasing p21/Cip1/Waf1 levels, thus blocking activation of Cdk2. That results in Rb hypophosphorylation and blockage of the G1/S transition. Separately, ATM also phosphorylates and activates Chk1, which phosphorylates Cdc25C. This inactivates Cdc25C and prevents it from dephosphorylating the inhibitory phosphotyrosine residue on cdc2/Cdk1, thus preventing the G2/M transition. The complex phenotype of cells derived from patients with AT suggests that ATM has additional cellular substrates. In unirradiated cells, ATM is present as an inactive homodimer or multimer. Double-stranded breaks in DNA caused by ionizing radiation cause rapid ATM kinase activation through dissociation of this complex and ATM autophosphorylation at Ser1981.
Specificità
Detects ATM.
Immunogeno
Epitope: Abl-interacting domain
KLH-conjugated synthetic peptide corresponding to sequence derived from the Abl-interacting domain of human ATM.
Applicazioni
Anti-ATM Antibody detects level of ATM & has been published & validated for use in WB & IF.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
Nuclear Receptors
Western Blotting Analysis: A 1:5,000 dilution from a representative lot detected ATM in 10 µg of HeLa nuclear extract.
Immunocytochemistry Analysis: A representative lot detected ATM in HeLa and NIH/3T3 cells.
Immunocytochemistry Analysis: 2 µg/mL from a representative lot detected ATM in NIH/3T3 cells.
Immunocytochemistry Analysis: A representative lot detected ATM in HeLa and NIH/3T3 cells.
Immunocytochemistry Analysis: 2 µg/mL from a representative lot detected ATM in NIH/3T3 cells.
Qualità
Evaluated by Western Blotting in HeLa nuclear extract.
Western Blotting Analysis: A 1:500 dilution of this antibody detected ATM in 10 µg of HeLa nuclear extract.
Western Blotting Analysis: A 1:500 dilution of this antibody detected ATM in 10 µg of HeLa nuclear extract.
Descrizione del bersaglio
>260 kDa observed. Uncharacterized band(s) may appear in some lysates.
Stato fisico
Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Stoccaggio e stabilità
Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
Risultati analitici
Control
HeLa nuclear extract
HeLa nuclear extract
Altre note
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Note legali
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Raccomandato
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Differential roles for checkpoint kinases in DNA damage-dependent degradation of the Cdc25A protein phosphatase.
Jin, J; Ang, XL; Ye, X; Livingstone, M; Harper, JW
The Journal of Biological Chemistry null
Regulation of intra-S phase checkpoint by ionizing radiation (IR)-dependent and IR-independent phosphorylation of SMC3.
Luo, H; Li, Y; Mu, JJ; Zhang, J; Tonaka, T; Hamamori, Y; Jung, SY; Wang, Y; Qin, J
The Journal of Biological Chemistry null
Aloke Sarkar et al.
Haematologica (2020-02-08)
Ataxia telangiectasia mutated (ATM), a critical DNA damage sensor with protein kinase activity,is frequently altered in human cancers including mantle cell lymphoma (MCL). Loss of ATM protein is linked to accumulation of nonfunctional mitochondria and defective mitophagy, in both murine
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