06-1038
Anti-Unc84B/SUN2 Antibody
0.5 mg/mL, from rabbit
Sinonimo/i:
Rab5-interacting protein, Sad1 unc-84 domain protein 2, Sad1/unc-84 protein-like 2, nuclear envelope protein, unc-84 homolog B, unc-84 homolog B (C. elegans)
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
polyclonal
application:
IP
WB
WB
Reattività contro le specie:
human, canine
tecniche:
immunoprecipitation (IP): suitable
western blot: suitable
western blot: suitable
citations:
13
Prodotti consigliati
Origine biologica
rabbit
Forma dell’anticorpo
affinity isolated antibody
Tipo di anticorpo
primary antibodies
Clone
polyclonal
Purificato mediante
affinity chromatography
Reattività contro le specie
human, canine
Reattività contro le specie (prevista in base all’omologia)
mouse (87%), dog, horse, rat (87%)
Concentrazione
0.5 mg/mL
tecniche
immunoprecipitation (IP): suitable
western blot: suitable
Isotipo
IgG
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
wet ice
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... SUN2(25777)
Descrizione generale
UNC84B (Protein UNC-84 homolog B or SUN2), is a single-pass nuclear envelope transmembrane protein responsible for nuclear migrations that are essential for development. UNC-84B has a predicted transmembrane domain and a C-terminal region with similarity to the S. pombe spindle pole body protein Sad1. UNC-84B interacts with RAB5A and is widely expressed, and highly expressed in the heart, lungs and muscles. UNC-84 may function to facilitate a nuclear-centrosomal interaction required for nuclear migration and anchorage.
Specificità
This antibody recognizes Unc84B/SUN2 at the N-terminus.
Immunogeno
Epitope: N-terminus
KLH-conjugated linear peptide corresponding to human Unc84B/SUN2 at and around the N-terminus.
Applicazioni
Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated Unc84B/SUN2 from 500 µg of U2OS cell lysate.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
This Anti-Unc84B/SUN2 Antibody is validated for use in Immunoprecipitation and Western Blotting for the detection of Unc84B/SUN2.
Qualità
Evaluated by Western Blotting in U2OS cell lysate.
Western Blot (SNAP ID) Analysis: 1:250 dilution of this antibody detected Unc84B/SUN2 on 10 µg of U2OS cell lysate.
Western Blot (SNAP ID) Analysis: 1:250 dilution of this antibody detected Unc84B/SUN2 on 10 µg of U2OS cell lysate.
Descrizione del bersaglio
~80 kDa
Stato fisico
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Stoccaggio e stabilità
Stable for 1 year at 2-8°C from date of receipt.
Risultati analitici
Control
U2OS cell lysate.
U2OS cell lysate.
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
The leukocyte nuclear envelope proteome varies with cell activation and contains novel transmembrane proteins that affect genome architecture.
Korfali, N; Wilkie, GS; Swanson, SK; Srsen, V; Batrakou, DG; Fairley, EA; Malik et al.
Molecular and Cellular Proteomics null
Nadia Korfali et al.
Methods in molecular biology (Clifton, N.J.), 1411, 3-44 (2016-05-06)
Nuclei can be relatively easily extracted from homogenized liver due to the softness of the tissue and crudely separated from other cellular organelles by low-speed centrifugation due to the comparatively large size of nuclei. However, further purification is complicated by
Nanami Ueda et al.
Frontiers in cell and developmental biology, 10, 885859-885859 (2022-06-07)
The linker of nucleoskeleton and cytoskeleton (LINC) complex is composed of the inner nuclear membrane-spanning SUN proteins and the outer nuclear membrane-spanning nesprin proteins. The LINC complex physically connects the nucleus and plasma membrane via the actin cytoskeleton to perform
Nikolaj Zuleger et al.
Genome biology, 14(2), R14-R14 (2013-02-19)
Different cell types have distinctive patterns of chromosome positioning in the nucleus. Although ectopic affinity-tethering of specific loci can be used to relocate chromosomes to the nuclear periphery, endogenous nuclear envelope proteins that control such a mechanism in mammalian cells
Nadia Korfali et al.
Nucleus (Austin, Tex.), 3(6), 552-564 (2012-09-20)
One hypothesis to explain how mutations in the same nuclear envelope proteins yield pathologies focused in distinct tissues is that as yet unidentified tissue-specific partners mediate the disease pathologies. The nuclear envelope proteome was recently determined from leukocytes and muscle.
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