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Key Documents

05-902R

Sigma-Aldrich

Anti-HA Tag Antibody, clone 114-2C-7, rabbit monoclonal

clone 114-2C-7, Upstate®, from rabbit

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.43

Origine biologica

rabbit

Livello qualitativo

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

114-2C-7, monoclonal

Reattività contro le specie (prevista in base all’omologia)

all

Produttore/marchio commerciale

Upstate®

tecniche

western blot: suitable

Isotipo

IgG

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Descrizione generale

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule that corresponds to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. 05-902R is a recombinant version of the rabbit monoclonal Cat no. 05-902.

Specificità

The antibody recognizes recombinant proteins containing the HA epitope tag.

Immunogeno

Synthetic peptide containing the sequence ...CYPYDVPDY... derived from the influenza virus hemaglutinin (HA) protein.

Applicazioni

Anti-HA Tag Antibody, clone 114-2C-7 detects level of HA Tag & has been published & validated for use in WB.
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags

Qualità

Routinely evaluated by Western Blot HA-tagged WNT-1 transfected HEK293 cells.

Stato fisico

Format: Purified
Protein A purified
Protein A purified rabbit immunoglobulin in buffer containing 60mM Tris, 90 mM NaCl, 0.03% NaN3, 60mM glycine, pH 7.5, and 40% glycerol.

Stoccaggio e stabilità

Maintain for 1 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note legali

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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Manuel Osorio-Valeriano et al.
Molecular cell, 81(19), 3992-4007 (2021-09-26)
ParB-like CTPases mediate the segregation of bacterial chromosomes and low-copy number plasmids. They act as DNA-sliding clamps that are loaded at parS motifs in the centromere of target DNA molecules and spread laterally to form large nucleoprotein complexes serving as
Dario Zimmerli et al.
eLife, 9 (2020-08-19)
BCL9 and PYGO are β-catenin cofactors that enhance the transcription of Wnt target genes. They have been proposed as therapeutic targets to diminish Wnt signaling output in intestinal malignancies. Here we find that, in colorectal cancer cells and in developing
Meng-Ling Wu et al.
Cell communication and signaling : CCS, 12, 22-22 (2014-03-29)
Vascular smooth muscle cells (VSMCs) of the arterial wall play a critical role in the development of occlusive vascular diseases. Cysteine-rich protein 2 (CRP2) is a VSMC-expressed LIM-only protein, which functionally limits VSMC migration and protects against pathological vascular remodeling.
Jing Xu et al.
PloS one, 7(12), e52799-e52799 (2012-12-20)
Conjugation in Tetrahymena thermophila involves a developmental program consisting of three prezygotic nuclear divisions, pronuclear exchange and fusion, and postzygotic and exconjugant stages. The conjugation junction structure appears during the initiation of conjugation development, and disappears during the exconjugant stage.
Chengwen Wei et al.
Neuroscience bulletin, 38(3), 249-262 (2021-12-17)
The radial migration of cortical pyramidal neurons (PNs) during corticogenesis is necessary for establishing a multilayered cerebral cortex. Neuronal migration defects are considered a critical etiology of neurodevelopmental disorders, including autism spectrum disorders (ASDs), schizophrenia, epilepsy, and intellectual disability (ID).

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