05-636-AF647
Anti-phospho Histone H2A.X (Ser139) Antibody, clone JBW301, Alexa Fluor™ 647
clone JBW301, 0.5 mg/mL, from mouse
Sinonimo/i:
Histone H2AX, H2a/x, Histone H2A.X
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
Coniugato
ALEXA FLUOR™ 647
Forma dell’anticorpo
purified antibody
Tipo di anticorpo
primary antibodies
Clone
JBW301, monoclonal
Reattività contro le specie
human
Reattività contro le specie (prevista in base all’omologia)
vertebrates (based on 100% sequence homology)
Concentrazione
0.5 mg/mL
tecniche
immunocytochemistry: suitable
Isotipo
IgG1
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
wet ice
modifica post-traduzionali bersaglio
phosphorylation (pSer139)
Informazioni sul gene
human ... H2AX(3014)
Descrizione generale
As a member of the histone H2A family, histone H2A.x (H2A.x) is a variant histone H2A which replaces conventional H2A in a subset of nucleosomes. H2A.x is involved in the DNA repair of double-strand breakage (DSB) damage on nuclear DNA. After a double strand DNA break, H2A.x is rapidly phosphorylated at serine 139 by ATM kinase and becomes gamma-H2AFX. This phosphorylation step can extend up to several thousand nucleosomes from the actual site of the DSB and may mark the surrounding chromatin for recruitment of proteins required for DNA damage signaling and repair. As a part of posttranslational modifications during apoptosis caused by severe DNA damage, high expression of phosphorylated H2A.x is considered as an accurate indicator of apoptosis.
Immunogeno
Linear peptide corresponding to phospho Histone H2A.X (Ser139).
Applicazioni
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
This Anti-phospho Histone H2A.X (Ser139) Antibody, clone JBW301, Alexa Fluor™ 647 is validated for use in ICC for the detection of phospho Histone H2A.X (Ser139).
Qualità
Evaluated by Immunocytochemistry in untreated and staurosporin treated HeLa cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected phospho Histone H2A.X (Ser139) in staurosporin treated HeLa cells.
Alexa Fluor™ is a registered trademark of Life Technologies.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected phospho Histone H2A.X (Ser139) in staurosporin treated HeLa cells.
Alexa Fluor™ is a registered trademark of Life Technologies.
Descrizione del bersaglio
~17 kDa observed. Refer to Cat. No. 05-636 for observed molecular weight information.
Stato fisico
Protein G Purified
Purified mouse conjugate in buffer containing PBS, 15 mg/ml BSA and 0.1% sodium azide.
Stoccaggio e stabilità
Stable for 1 year at 2-8°C from date of receipt.
Note legali
ALEXA FLUOR is a trademark of Life Technologies
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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