04-973

Anti-CD39 Antibody, clone 5F2

clone 5F2, from mouse

• Sinonimo/i: ectonucleoside triphosphate diphosphohydrolase 1, ATPDase, ecto-apyrase, NTPDase-1, Ecto-ATP diphosphohydrolase, Lymphoid cell activation antigen, NTPDase 1, Ecto-apyrase, CD39 antigen
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: 5F2, monoclonal
• Reattività contro le specie: mouse
• tecniche: flow cytometry: suitable; western blot: suitable
• Isotipo: IgG1κ
• N° accesso NCBI: NP_033978
• N° accesso UniProt: P55772
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: mouse ... Entpd1(12495)

Descrizione

Descrizione generale

Ectonucleoside triphosphate diphosphohydrolase 1 (NTDase 1 or CD39) belongs to the membrane-bound ecto-nucleoside triphosphates diphosphohydrolase family (E-NTPDases) that are characterized for their ability to hydrolyze nucleoside di- and triphosphates, such as ATP, in the presence of cations, i.e. CA2+ and Mg2+. CD39 is an important membrane protein containing two transmembrane domains and a large NTPDase active, extracellular region containing apyrase conserved regions. It is heavily glycosolated, having six known N-glycosolation sites, and functions on the plasma membrane’s outer surface. CD39 initiates the metabolism, of ATP and ADP to adenosine through a 2-step exzymatic reaction, providing extracellular adenosine for the purpose of regulating various inflammatory endpoints. It is a putative marker for B-cell activation and its expression has also been observed on NK-cells, dendritic cells, T-cells, and vascular endothelial cells. CD39 is a known inhibitor of ADP-induced platelet aggregation and may also play a role in angiogenesis.

Specificità

This antibody recognizes CD39.

Immunogeno

Recombinant protein corresponding to mouse CD39.

Applicazioni

This Anti-CD39 Antibody, clone 5F2 is validated for use in FC, WB for the detection of CD39.

Western Blot Analysis: A previous lot was used by an independent laboratory in WB.

Qualità

Evaluated by Flow Cytometry in BALB/c splenocytes.

Flow Cytometry Analysis: 0.1 µg/mL of this antibody detected CD39 in BALB/c splenocytes.

Descrizione del bersaglio

~ 100 kDa
It has been published that CD39 shows molecular weights between ~70-100 kDa (Wang, T.F. et al., 1996).

Stato fisico

Format: Purified

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable